Serum Has Higher Proportion of Janus Kinase 2 V617F Mutation Compared to Paired EDTA-Whole Blood Sample: A Model for Somatic Mutation Quantification Using qPCR and the 2(-∆∆Cq) Method

Detection of the Janus Kinase-2 (JAK2) V617F mutation is a diagnostic criterion for myeloproliferative neoplasms, and high levels of mutant alleles are associated with worse outcomes. This mutation is usually tested on blood DNA by allele-specific qPCR (AS-qPCR) and measured using absolute quantific...

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Detalles Bibliográficos
Autores principales: Barra, Gustavo Barcelos, Santa Rita, Ticiane Henriques, Almeida, Ana Luisa Santa Cruz, Jácomo, Rafael Henriques, Nery, Lídia Freire Abdalla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151023/
https://www.ncbi.nlm.nih.gov/pubmed/32178286
http://dx.doi.org/10.3390/diagnostics10030153

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151023/
https://www.ncbi.nlm.nih.gov/pubmed/32178286
http://dx.doi.org/10.3390/diagnostics10030153

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