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Quantification of Pneumocystis jirovecii: Cross-Platform Comparison of One qPCR Assay with Leading Platforms and Six Master Mixes

Diagnosis of Pneumocystis jirovecii pneumonia relies on nucleic acid quantification in respiratory samples. Lack of standardization among molecular assays results in significant differences among assays/centers. To further promote standardization, we compared four thermocyclers and six master mixes...

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Detalles Bibliográficos
Autores principales: Dellière, Sarah, Gits-Muselli, Maud, White, P. Lewis, Mengoli, Carlo, Bretagne, Stéphane, Alanio, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151141/
https://www.ncbi.nlm.nih.gov/pubmed/31888050
http://dx.doi.org/10.3390/jof6010009