Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid

BACKGROUND: Keloid is an excessive fibrosis disease caused by the abnormal proliferation of collagen fibers following trauma. Previous studies have shown that genetic factors have been considered to play important roles in keloid formation. This study is aimed to investigate the regulatory network o...

Descripción completa

Detalles Bibliográficos
Autores principales: Duan, Xilei, Wu, Yuemeng, Zhang, Zheng, Lu, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154391/
https://www.ncbi.nlm.nih.gov/pubmed/32309369
http://dx.doi.org/10.21037/atm.2020.01.07
_version_ 1783521806429192192
author Duan, Xilei
Wu, Yuemeng
Zhang, Zheng
Lu, Zhong
author_facet Duan, Xilei
Wu, Yuemeng
Zhang, Zheng
Lu, Zhong
author_sort Duan, Xilei
collection PubMed
description BACKGROUND: Keloid is an excessive fibrosis disease caused by the abnormal proliferation of collagen fibers following trauma. Previous studies have shown that genetic factors have been considered to play important roles in keloid formation. This study is aimed to investigate the regulatory network of messenger RNAs (mRNAs) microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in keloid, and identifying its key biomarkers METHODS: We performed RNA-seq and miRNA-seq on keloid and normal skin samples. Sequencing datasets were analyzed by bioinformatics. Gene ontology (GO) and pathway analysis presented the characteristics of associated protein-coding genes. Differentially expressed ceRNAs were validated by quantitative reverse transcriptase-PCR (qRT-PCR). RESULTS: We identified a total of 319 lncRNAs, 1,533 mRNAs and 40 miRNAs as keloid-specific RNAs. Both the GO biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were analyzed for 1,219 specific genes with differentially expressed mRNAs. Then, with 509 key lncRNAs, 25 miRNAs, and 94 mRNAs, we constructed a ceRNA network and explored any potential underlying mechanisms. In the regulation of the actin cytokeleton pathway, we validated 2 pairs of ceRNAs EGFR/miR-370-3p/lnc-GLB1L-1 and ITGB5/ miR-204/ lnc-CASP9-3 in another sample size in keloid. CONCLUSIONS: Through RNA-seq and miRNA-seq, we identified keloid-associated lncRNAs, mRNAs and miRNAs, which can be used as potential therapeutic targets and biomarkers for keloid. Our study may lay a foundation for future pathogenesis studies.
format Online
Article
Text
id pubmed-7154391
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher AME Publishing Company
record_format MEDLINE/PubMed
spelling pubmed-71543912020-04-17 Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid Duan, Xilei Wu, Yuemeng Zhang, Zheng Lu, Zhong Ann Transl Med Original Article BACKGROUND: Keloid is an excessive fibrosis disease caused by the abnormal proliferation of collagen fibers following trauma. Previous studies have shown that genetic factors have been considered to play important roles in keloid formation. This study is aimed to investigate the regulatory network of messenger RNAs (mRNAs) microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in keloid, and identifying its key biomarkers METHODS: We performed RNA-seq and miRNA-seq on keloid and normal skin samples. Sequencing datasets were analyzed by bioinformatics. Gene ontology (GO) and pathway analysis presented the characteristics of associated protein-coding genes. Differentially expressed ceRNAs were validated by quantitative reverse transcriptase-PCR (qRT-PCR). RESULTS: We identified a total of 319 lncRNAs, 1,533 mRNAs and 40 miRNAs as keloid-specific RNAs. Both the GO biological processes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were analyzed for 1,219 specific genes with differentially expressed mRNAs. Then, with 509 key lncRNAs, 25 miRNAs, and 94 mRNAs, we constructed a ceRNA network and explored any potential underlying mechanisms. In the regulation of the actin cytokeleton pathway, we validated 2 pairs of ceRNAs EGFR/miR-370-3p/lnc-GLB1L-1 and ITGB5/ miR-204/ lnc-CASP9-3 in another sample size in keloid. CONCLUSIONS: Through RNA-seq and miRNA-seq, we identified keloid-associated lncRNAs, mRNAs and miRNAs, which can be used as potential therapeutic targets and biomarkers for keloid. Our study may lay a foundation for future pathogenesis studies. AME Publishing Company 2020-03 /pmc/articles/PMC7154391/ /pubmed/32309369 http://dx.doi.org/10.21037/atm.2020.01.07 Text en 2020 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Duan, Xilei
Wu, Yuemeng
Zhang, Zheng
Lu, Zhong
Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid
title Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid
title_full Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid
title_fullStr Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid
title_full_unstemmed Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid
title_short Identification and analysis of dysregulated lncRNA and associated ceRNA in the pathogenesis of keloid
title_sort identification and analysis of dysregulated lncrna and associated cerna in the pathogenesis of keloid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154391/
https://www.ncbi.nlm.nih.gov/pubmed/32309369
http://dx.doi.org/10.21037/atm.2020.01.07
work_keys_str_mv AT duanxilei identificationandanalysisofdysregulatedlncrnaandassociatedcernainthepathogenesisofkeloid
AT wuyuemeng identificationandanalysisofdysregulatedlncrnaandassociatedcernainthepathogenesisofkeloid
AT zhangzheng identificationandanalysisofdysregulatedlncrnaandassociatedcernainthepathogenesisofkeloid
AT luzhong identificationandanalysisofdysregulatedlncrnaandassociatedcernainthepathogenesisofkeloid