Cargando…

The Effect of Recombinant Tags on Citrus paradisi Flavonol-Specific 3-O Glucosyltransferase Activity

Recombinant tags are used extensively in protein expression systems to allow purification through IMAC (Immobilized Metal Affinity Chromatography), identification through Western blot, and to facilitate crystal formation for structural analysis. While widely used, their role in enzyme characterizati...

Descripción completa

Detalles Bibliográficos
Autores principales: Birchfield, Aaron S., McIntosh, Cecilia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154896/
https://www.ncbi.nlm.nih.gov/pubmed/32213838
http://dx.doi.org/10.3390/plants9030402