Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica

Isotopic labeling experiments performed with a newly identified bacterial trichoacorenol synthase established a 1,5‐hydride shift occurring in the cyclization mechanism. During EI‐MS analysis, major fragments of the sesquiterpenoid were shown to arise via cryptic hydrogen movements. Therefore, the i...

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Detalles Bibliográficos
Autores principales: Rinkel, Jan, Dickschat, Jeroen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155024/
https://www.ncbi.nlm.nih.gov/pubmed/31553510
http://dx.doi.org/10.1002/cbic.201900584
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author Rinkel, Jan
Dickschat, Jeroen S.
author_facet Rinkel, Jan
Dickschat, Jeroen S.
author_sort Rinkel, Jan
collection PubMed
description Isotopic labeling experiments performed with a newly identified bacterial trichoacorenol synthase established a 1,5‐hydride shift occurring in the cyclization mechanism. During EI‐MS analysis, major fragments of the sesquiterpenoid were shown to arise via cryptic hydrogen movements. Therefore, the interpretation of earlier results regarding the cyclization mechanism obtained by feeding experiments in Trichoderma is revised.
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spelling pubmed-71550242020-04-15 Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica Rinkel, Jan Dickschat, Jeroen S. Chembiochem Communications Isotopic labeling experiments performed with a newly identified bacterial trichoacorenol synthase established a 1,5‐hydride shift occurring in the cyclization mechanism. During EI‐MS analysis, major fragments of the sesquiterpenoid were shown to arise via cryptic hydrogen movements. Therefore, the interpretation of earlier results regarding the cyclization mechanism obtained by feeding experiments in Trichoderma is revised. John Wiley and Sons Inc. 2019-11-07 2020-03-16 /pmc/articles/PMC7155024/ /pubmed/31553510 http://dx.doi.org/10.1002/cbic.201900584 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Rinkel, Jan
Dickschat, Jeroen S.
Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica
title Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica
title_full Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica
title_fullStr Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica
title_full_unstemmed Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica
title_short Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica
title_sort mechanistic studies on trichoacorenol synthase from amycolatopsis benzoatilytica
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155024/
https://www.ncbi.nlm.nih.gov/pubmed/31553510
http://dx.doi.org/10.1002/cbic.201900584
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