Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ

Lipid II precursor and its processing by a flippase and peptidoglycan polymerases are considered key hot spot targets for antibiotics. We have developed a fluorescent anisotropy (FA) assay using a unique and versatile probe (fluorescent lipid II) and monitored direct binding between lipid II and int...

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Detalles Bibliográficos
Autores principales: Boes, Adrien, Olatunji, Samir, Mohammadi, Tamimount, Breukink, Eefjan, Terrak, Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156629/
https://www.ncbi.nlm.nih.gov/pubmed/32286439
http://dx.doi.org/10.1038/s41598-020-63380-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156629/
https://www.ncbi.nlm.nih.gov/pubmed/32286439
http://dx.doi.org/10.1038/s41598-020-63380-2

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