Generating homogenous cortical preplate and deep-layer neurons using a combination of 2D and 3D differentiation cultures

Embryonic stem cells (ESCs) can be used to derive different neural subtypes. Current differentiation protocols generate heterogeneous neural subtypes rather than a specific neuronal population. Here, we present a protocol to derive separate two-deep layer cortical neurons from mouse ESCs (mESCs). mE...

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Detalles Bibliográficos
Autores principales: Alsanie, Walaa F., Bahri, Ola A., Habeeballah, Hamza H., Alhomrani, Majid, Almehmadi, Mazen M., Alsharif, Khalaf, Felemban, Ebaa M., Althobaiti, Yusuf S., Almalki, Atiah H., Alsaab, Hashem O., Gaber, Ahmed, Hassan, Mohamed M., Hardy, Ana Maria Gregio, Alhadidi, Qasim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156727/
https://www.ncbi.nlm.nih.gov/pubmed/32286346
http://dx.doi.org/10.1038/s41598-020-62925-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156727/
https://www.ncbi.nlm.nih.gov/pubmed/32286346
http://dx.doi.org/10.1038/s41598-020-62925-9

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