Cargando…

Generating homogenous cortical preplate and deep-layer neurons using a combination of 2D and 3D differentiation cultures

Embryonic stem cells (ESCs) can be used to derive different neural subtypes. Current differentiation protocols generate heterogeneous neural subtypes rather than a specific neuronal population. Here, we present a protocol to derive separate two-deep layer cortical neurons from mouse ESCs (mESCs). mE...

Descripción completa

Detalles Bibliográficos
Autores principales:	Alsanie, Walaa F., Bahri, Ola A., Habeeballah, Hamza H., Alhomrani, Majid, Almehmadi, Mazen M., Alsharif, Khalaf, Felemban, Ebaa M., Althobaiti, Yusuf S., Almalki, Atiah H., Alsaab, Hashem O., Gaber, Ahmed, Hassan, Mohamed M., Hardy, Ana Maria Gregio, Alhadidi, Qasim
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156727/ https://www.ncbi.nlm.nih.gov/pubmed/32286346 http://dx.doi.org/10.1038/s41598-020-62925-9

Ejemplares similares

Pregabalin: Potential for Addiction and a Possible Glutamatergic Mechanism
por: Althobaiti, Yusuf S., et al.
Publicado: (2019)

Potential Benefits of N-Acetylcysteine in Preventing Pregabalin-Induced Seeking-Like Behavior
por: Almalki, Atiah H., et al.
Publicado: (2021)

Gabapentin-induced drug-seeking-like behavior: a potential role for the dopaminergic system
por: Althobaiti, Yusuf S., et al.
Publicado: (2020)

Sex differences in pregabalin-seeking like behavior in a conditioned place preference paradigm
por: Alsaab, Hashem O., et al.
Publicado: (2020)

Involvement of the dopaminergic system in the reward-related behavior of pregabalin
por: Althobaiti, Yusuf S., et al.
Publicado: (2021)

The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain
por: Alsanie, Walaa F., et al.
Publicado: (2023)

Nanomaterials for Antiangiogenic Therapies for Cancer: A Promising Tool for Personalized Medicine
por: Alsaab, Hashem O., et al.
Publicado: (2021)

Application of Three Ecological Assessment Tools in Examining Chromatographic Methods for the Green Analysis of a Mixture of Dopamine, Serotonin, Glutamate and GABA: A Comparative Study
por: Almalki, Atiah H., et al.
Publicado: (2021)

Progress in Clinical Trials of Photodynamic Therapy for Solid Tumors and the Role of Nanomedicine
por: Alsaab, Hashem O., et al.
Publicado: (2020)

Perception of Threat and Psychological Impact of COVID-19 among Expatriates in Makkah Region, Saudi Arabia
por: Algarni, Majed A., et al.
Publicado: (2021)

Enhancing the Antipsychotic Effect of Risperidone by Increasing Its Binding Affinity to Serotonin Receptor via Picric Acid: A Molecular Dynamics Simulation
por: Alhomrani, Majid, et al.
Publicado: (2022)

Malaria Prevalence in a Low Transmission Area, Jazan District of Southwestern Saudi Arabia
por: Hawash, Yousry, et al.
Publicado: (2019)

Prenatal Exposure to Gabapentin Alters the Development of Ventral Midbrain Dopaminergic Neurons
por: Alsanie, Walaa F., et al.
Publicado: (2022)

The Effects of Prenatal Exposure to Pregabalin on the Development of Ventral Midbrain Dopaminergic Neurons
por: Alsanie, Walaa F., et al.
Publicado: (2022)

Increasing the Efficacy of Seproxetine as an Antidepressant Using Charge–Transfer Complexes
por: Alsanie, Walaa F., et al.
Publicado: (2022)

Multispectral and Molecular Docking Studies Reveal Potential Effectiveness of Antidepressant Fluoxetine by Forming π-Acceptor Complexes
por: Gaber, Ahmed, et al.
Publicado: (2022)

Attempting to Increase the Effectiveness of the Antidepressant Trazodone Hydrochloride Drug Using π-Acceptors
por: Alsanie, Walaa F., et al.
Publicado: (2022)

Analysis of Preplate Splitting and Early Cortical Development Illuminates the Biology of Neurological Disease
por: Olson, Eric C.
Publicado: (2014)

The Influence of Prenatal Exposure to Quetiapine Fumarate on the Development of Dopaminergic Neurons in the Ventral Midbrain of Mouse Embryos
por: Alsanie, Walaa F., et al.
Publicado: (2022)

Enhancement of Haloperidol Binding Affinity to Dopamine Receptor via Forming a Charge-Transfer Complex with Picric Acid and 7,7,8,8-Tetracyanoquinodimethane for Improvement of the Antipsychotic Efficacy
por: Alamri, Abdulhakeem S., et al.
Publicado: (2022)

A Comparative Study of Magnetic-Activated Cell Sorting, Cytotoxicity and Preplating for the Purification of Human Myoblasts
por: Park, Yoon Ghil, et al.
Publicado: (2006)

The Possible Relationship between the Abuse of Tobacco, Opioid, or Alcohol with COVID-19
por: Althobaiti, Yusuf S., et al.
Publicado: (2020)

Effects of Ceftriaxone on Glial Glutamate Transporters in Wistar Rats Administered Sequential Ethanol and Methamphetamine
por: Althobaiti, Yusuf S., et al.
Publicado: (2016)

Cardioprotective Potential of Garlic Oil and Its Active Constituent, Diallyl Disulphide, in Presence of Carvedilol during Chronic Isoprenaline Injection-Mediated Myocardial Necrosis in Rats
por: Asdaq, Syed Mohammed Basheeruddin, et al.
Publicado: (2021)

Targeted ablation and reorganization of the principal preplate neurons and their neuroblasts identified by golli promoter transgene expression in the neocortex of mice
por: Xie, Yuan-Yun, et al.
Publicado: (2009)

The Psychological Impact of COVID-19 on Healthcare Workers in Saudi Arabia: A Year Later Into the Pandemic
por: Almalki, Atiah H., et al.
Publicado: (2021)

Growth defects in the dorsal pallium after genetically targeted ablation of principal preplate neurons and neuroblasts: a morphometric analysis
por: Fisher, Robin, et al.
Publicado: (2010)

Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed D. melanogaster (Oregon R(+)): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters
por: Shabir, Shabnam, et al.
Publicado: (2023)

Antidepressant Effect of Crocin in Mice with Chronic Mild Stress
por: Alsanie, Walaa F., et al.
Publicado: (2022)

Potential benefits of gallic acid as skeletal muscle relaxant in animal experimental models
por: Asdaq, Syed Mohammed Basheeruddin, et al.
Publicado: (2021)

New Mononuclear and Binuclear Cu(II), Co(II), Ni(II), and Zn(II) Thiosemicarbazone Complexes with Potential Biological Activity: Antimicrobial and Molecular Docking Study
por: Gaber, Ahmed, et al.
Publicado: (2021)

Integrative genomic analysis of early neurogenesis reveals a temporal genetic program for differentiation and specification of preplate and Cajal-Retzius neurons
por: Li, Jia, et al.
Publicado: (2021)

Lrp12/Mig13a Reveals Changing Patterns of Preplate Neuronal Polarity during Corticogenesis that Are Absent in Reeler Mutant Mice
por: Schneider, Stephanie, et al.
Publicado: (2011)

The Potential Benefits of Using Garlic Oil and Its Active Constituent, Dially Disulphide, in Combination With Carvedilol in Ameliorating Isoprenaline-Induced Cardiac Damage in Rats
por: Asdaq, Syed Mohammed Basheeruddin, et al.
Publicado: (2021)

Characterization of Phytochemicals, Nutrients, and Antiradical Potential in Slim Amaranth
por: Sarker, Umakanta, et al.
Publicado: (2022)

Satisfaction of psychologically impaired patients with health-care services: A Saudi Arabian perspective
por: Alhomrani, Majid, et al.
Publicado: (2022)

Benchmarking of ATAC Sequencing Data From BGI’s Low-Cost DNBSEQ-G400 Instrument for Identification of Open and Occupied Chromatin Regions
por: Naval-Sanchez, Marina, et al.
Publicado: (2022)

Vitamin D deficiency in children living in Jeddah, Saudi Arabia
por: Mansour, Maha M. H. K., et al.
Publicado: (2012)

Spectroscopic and Molecular Docking Studies of Cu(II), Ni(II), Co(II), and Mn(II) Complexes with Anticonvulsant Therapeutic Agent Gabapentin
por: Refat, Moamen S., et al.
Publicado: (2022)

Gene Expression Profiling of Preplate Neurons Destined for the Subplate: Genes Involved in Transcription, Axon Extension, Neurotransmitter Regulation, Steroid Hormone Signaling, and Neuronal Survival
por: Osheroff, Hilleary, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_248r9l8samf3des07ns3o2na2m