Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers

Selectable markers are indispensable for genetic engineering, yet their number and variety are limited. Most selection procedures for prototrophic cells rely on the introduction of antibiotic resistance genes. New minimally invasive tools are needed to facilitate sophisticated genetic manipulations....

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Detalles Bibliográficos
Autores principales: Birštonas, Lukas, Dallemulle, Alex, López-Berges, Manuel S., Jacobsen, Ilse D., Offterdinger, Martin, Abt, Beate, Straßburger, Maria, Bauer, Ingo, Schmidt, Oliver, Sarg, Bettina, Lindner, Herbert, Haas, Hubertus, Gsaller, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157766/
https://www.ncbi.nlm.nih.gov/pubmed/32265325
http://dx.doi.org/10.1128/mBio.00230-20

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157766/
https://www.ncbi.nlm.nih.gov/pubmed/32265325
http://dx.doi.org/10.1128/mBio.00230-20

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