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Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4

OBJECTIVES: To investigate the effect of lupeol in a mouse model of viral myocarditis induced by coxsackie virus B3 (CVB3). METHODS: Mice were separated into controls (DMEM, n = 20) and CVB3 infected groups (i.e., untreated CVB3 [n = 40]; CVB3 + lupeol 50 mg/kg [n = 40]; CVB3 + lupeol 100 mg/kg [n =...

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Autores principales: Xu, Ming, Li, Xiaoyong, Song, Laichun, Tao, Chao, Fang, Jihui, Tao, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158257/
https://www.ncbi.nlm.nih.gov/pubmed/32290748
http://dx.doi.org/10.1177/0300060520910908
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author Xu, Ming
Li, Xiaoyong
Song, Laichun
Tao, Chao
Fang, Jihui
Tao, Liang
author_facet Xu, Ming
Li, Xiaoyong
Song, Laichun
Tao, Chao
Fang, Jihui
Tao, Liang
author_sort Xu, Ming
collection PubMed
description OBJECTIVES: To investigate the effect of lupeol in a mouse model of viral myocarditis induced by coxsackie virus B3 (CVB3). METHODS: Mice were separated into controls (DMEM, n = 20) and CVB3 infected groups (i.e., untreated CVB3 [n = 40]; CVB3 + lupeol 50 mg/kg [n = 40]; CVB3 + lupeol 100 mg/kg [n = 40]; CVB3 + small interfering RNA (siRNA)- toll-like receptor 4 (TLR4) [n = 20]; siRNA + EXP-H mice [n = 20]). Reverse transcription polymerase chain reaction (RT-PCR), western-blot assay, immunohistochemistry, enzyme-linked immunosorbent (ELISA) assay and histopathology were performed to investigate the cardioprotective role of lupeol. RESULTS: The elevated pro-inflammatory cytokines in CVB3-infected mice (i.e., interleukin-1β [IL-1β]; interleukin-6 [IL-6]; tumour necrosis factor-α [TNF-α]) were significantly reduced by lupeol 50 or 100 mg/kg. Interestingly, the mRNA level and protein level of toll-like receptor 4 (TLR4) were inhibited by lupeol. CONCLUSIONS: Lupeol alleviates CVB3-induced viral myocarditis and myocardial damage in mice. The underlying mechanism may due to downregulation of TLR4.
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spelling pubmed-71582572020-04-20 Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4 Xu, Ming Li, Xiaoyong Song, Laichun Tao, Chao Fang, Jihui Tao, Liang J Int Med Res Pre-Clinical Research Report OBJECTIVES: To investigate the effect of lupeol in a mouse model of viral myocarditis induced by coxsackie virus B3 (CVB3). METHODS: Mice were separated into controls (DMEM, n = 20) and CVB3 infected groups (i.e., untreated CVB3 [n = 40]; CVB3 + lupeol 50 mg/kg [n = 40]; CVB3 + lupeol 100 mg/kg [n = 40]; CVB3 + small interfering RNA (siRNA)- toll-like receptor 4 (TLR4) [n = 20]; siRNA + EXP-H mice [n = 20]). Reverse transcription polymerase chain reaction (RT-PCR), western-blot assay, immunohistochemistry, enzyme-linked immunosorbent (ELISA) assay and histopathology were performed to investigate the cardioprotective role of lupeol. RESULTS: The elevated pro-inflammatory cytokines in CVB3-infected mice (i.e., interleukin-1β [IL-1β]; interleukin-6 [IL-6]; tumour necrosis factor-α [TNF-α]) were significantly reduced by lupeol 50 or 100 mg/kg. Interestingly, the mRNA level and protein level of toll-like receptor 4 (TLR4) were inhibited by lupeol. CONCLUSIONS: Lupeol alleviates CVB3-induced viral myocarditis and myocardial damage in mice. The underlying mechanism may due to downregulation of TLR4. SAGE Publications 2020-04-14 /pmc/articles/PMC7158257/ /pubmed/32290748 http://dx.doi.org/10.1177/0300060520910908 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Report
Xu, Ming
Li, Xiaoyong
Song, Laichun
Tao, Chao
Fang, Jihui
Tao, Liang
Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4
title Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4
title_full Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4
title_fullStr Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4
title_full_unstemmed Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4
title_short Lupeol alleviates coxsackievirus B3-induced viral myocarditis in mice via downregulating toll-like receptor 4
title_sort lupeol alleviates coxsackievirus b3-induced viral myocarditis in mice via downregulating toll-like receptor 4
topic Pre-Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158257/
https://www.ncbi.nlm.nih.gov/pubmed/32290748
http://dx.doi.org/10.1177/0300060520910908
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