Cargando…

Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation

PURPOSE: Bile acids play an important role in Clostridioides difficile life cycle. Deoxycholate (DCA), one of the most abundant secondary bile acids, is known to inhibit vegetative growth and toxin production. However, limited data are available on the role of DCA on C. difficile sporulation. Here,...

Descripción completa

Detalles Bibliográficos
Autores principales: Usui, Yukino, Ayibieke, Alafate, Kamiichi, Yuko, Okugawa, Shu, Moriya, Kyoji, Tohda, Shuji, Saito, Ryoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160582/
https://www.ncbi.nlm.nih.gov/pubmed/32322715
http://dx.doi.org/10.1016/j.heliyon.2020.e03717
_version_ 1783522785912422400
author Usui, Yukino
Ayibieke, Alafate
Kamiichi, Yuko
Okugawa, Shu
Moriya, Kyoji
Tohda, Shuji
Saito, Ryoichi
author_facet Usui, Yukino
Ayibieke, Alafate
Kamiichi, Yuko
Okugawa, Shu
Moriya, Kyoji
Tohda, Shuji
Saito, Ryoichi
author_sort Usui, Yukino
collection PubMed
description PURPOSE: Bile acids play an important role in Clostridioides difficile life cycle. Deoxycholate (DCA), one of the most abundant secondary bile acids, is known to inhibit vegetative growth and toxin production. However, limited data are available on the role of DCA on C. difficile sporulation. Here, we investigated the phenotypic and genotypic impact of DCA on the growth, toxin production, and sporulation of C. difficile. METHODOLOGY: Four genetically divergent C. difficile strains were cultured in nutrient-rich broth with and without DCA at various concentrations, and growth activity was evaluated for each strain. Cytotoxicity assays using culture supernatants from cells grown in nutrient-rich broth with and without 0.01% DCA were conducted. Sporulation efficiency was determined using sporulation media with and without 0.01% DCA. Transcript levels of tcdB and spo0A were analyzed using quantitative reverse-transcription polymerase chain reaction. RESULTS: We found that DCA led to growth reduction in a dose-depended manner and regulated toxin production by repressing tcdB expression during vegetative growth. To our knowledge, we have also provided the first evidence that DCA reduces C. difficile sporulation efficiency through the downregulation of spo0A expression during the sporulation stage. CONCLUSIONS: DCA modulates C. difficile sporulation, vegetative growth, and toxin production.
format Online
Article
Text
id pubmed-7160582
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-71605822020-04-22 Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation Usui, Yukino Ayibieke, Alafate Kamiichi, Yuko Okugawa, Shu Moriya, Kyoji Tohda, Shuji Saito, Ryoichi Heliyon Article PURPOSE: Bile acids play an important role in Clostridioides difficile life cycle. Deoxycholate (DCA), one of the most abundant secondary bile acids, is known to inhibit vegetative growth and toxin production. However, limited data are available on the role of DCA on C. difficile sporulation. Here, we investigated the phenotypic and genotypic impact of DCA on the growth, toxin production, and sporulation of C. difficile. METHODOLOGY: Four genetically divergent C. difficile strains were cultured in nutrient-rich broth with and without DCA at various concentrations, and growth activity was evaluated for each strain. Cytotoxicity assays using culture supernatants from cells grown in nutrient-rich broth with and without 0.01% DCA were conducted. Sporulation efficiency was determined using sporulation media with and without 0.01% DCA. Transcript levels of tcdB and spo0A were analyzed using quantitative reverse-transcription polymerase chain reaction. RESULTS: We found that DCA led to growth reduction in a dose-depended manner and regulated toxin production by repressing tcdB expression during vegetative growth. To our knowledge, we have also provided the first evidence that DCA reduces C. difficile sporulation efficiency through the downregulation of spo0A expression during the sporulation stage. CONCLUSIONS: DCA modulates C. difficile sporulation, vegetative growth, and toxin production. Elsevier 2020-04-13 /pmc/articles/PMC7160582/ /pubmed/32322715 http://dx.doi.org/10.1016/j.heliyon.2020.e03717 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Usui, Yukino
Ayibieke, Alafate
Kamiichi, Yuko
Okugawa, Shu
Moriya, Kyoji
Tohda, Shuji
Saito, Ryoichi
Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation
title Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation
title_full Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation
title_fullStr Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation
title_full_unstemmed Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation
title_short Impact of deoxycholate on Clostridioides difficile growth, toxin production, and sporulation
title_sort impact of deoxycholate on clostridioides difficile growth, toxin production, and sporulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160582/
https://www.ncbi.nlm.nih.gov/pubmed/32322715
http://dx.doi.org/10.1016/j.heliyon.2020.e03717
work_keys_str_mv AT usuiyukino impactofdeoxycholateonclostridioidesdifficilegrowthtoxinproductionandsporulation
AT ayibiekealafate impactofdeoxycholateonclostridioidesdifficilegrowthtoxinproductionandsporulation
AT kamiichiyuko impactofdeoxycholateonclostridioidesdifficilegrowthtoxinproductionandsporulation
AT okugawashu impactofdeoxycholateonclostridioidesdifficilegrowthtoxinproductionandsporulation
AT moriyakyoji impactofdeoxycholateonclostridioidesdifficilegrowthtoxinproductionandsporulation
AT tohdashuji impactofdeoxycholateonclostridioidesdifficilegrowthtoxinproductionandsporulation
AT saitoryoichi impactofdeoxycholateonclostridioidesdifficilegrowthtoxinproductionandsporulation