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DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection
BACKGROUND: Nanoparticles (NPs) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the DNA vaccination strategy against leptospirosis. OBJECTIVES: We evaluated the efficiency of halloysite clay nanotubes (HNTs) and amine-functionalised multi-walle...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164400/ https://www.ncbi.nlm.nih.gov/pubmed/32321154 http://dx.doi.org/10.1590/0074-02760190396 |
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author | Oliveira, Thaís Larré Bacelo, Kátia Leston Forster, Karine Maciel Ilha, Vinicius Rodrigues, Oscar Endrigo Hartwig, Daiane D |
author_facet | Oliveira, Thaís Larré Bacelo, Kátia Leston Forster, Karine Maciel Ilha, Vinicius Rodrigues, Oscar Endrigo Hartwig, Daiane D |
author_sort | Oliveira, Thaís Larré |
collection | PubMed |
description | BACKGROUND: Nanoparticles (NPs) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the DNA vaccination strategy against leptospirosis. OBJECTIVES: We evaluated the efficiency of halloysite clay nanotubes (HNTs) and amine-functionalised multi-walled carbon nanotubes (NH(2)-MWCNTs) in facilitating recombinant LemA antigen (rLemA) expression and protecting Golden Syrian hamsters (Mesocricetus auratus) against Leptospira interrogans lethal infection. METHODS: An indirect immunofluorescent technique was used to investigate the potency of HNTs and NH(2)-MWCNTs in enhancing the transfection and expression efficiency of the DNA vaccine in Chinese hamster ovary (CHO) cells. Hamsters were immunised with two doses of vaccines HNT-pTARGET/lemA, NH(2)-MWCNTs-pTARGET/lemA, pTARGET/lemA, and empty pTARGET (control), and the efficacy was determined in terms of humoral immune response and protection against a lethal challenge. FINDINGS: rLemA DNA vaccines carried by NPs were able to transfect CHO cells effectively, inducing IgG immune response in hamsters (p < 0.05), and did not exhibit cytotoxic effects. Furthermore, 83.3% of the hamsters immunised with NH(2)-MWCNTs-pTARGET/lemA were protected against the lethal challenge (p < 0.01), and 66.7% of hamsters immunised with HNT-pTARGET/lemA survived (p < 0.05). MAIN CONCLUSIONS: NH(2)-MWCNTs and HNTs can act as antigen carriers for mammalian cells and are suitable for DNA nanovaccine delivery. |
format | Online Article Text |
id | pubmed-7164400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-71644002020-04-23 DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection Oliveira, Thaís Larré Bacelo, Kátia Leston Forster, Karine Maciel Ilha, Vinicius Rodrigues, Oscar Endrigo Hartwig, Daiane D Mem Inst Oswaldo Cruz Original Article BACKGROUND: Nanoparticles (NPs) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the DNA vaccination strategy against leptospirosis. OBJECTIVES: We evaluated the efficiency of halloysite clay nanotubes (HNTs) and amine-functionalised multi-walled carbon nanotubes (NH(2)-MWCNTs) in facilitating recombinant LemA antigen (rLemA) expression and protecting Golden Syrian hamsters (Mesocricetus auratus) against Leptospira interrogans lethal infection. METHODS: An indirect immunofluorescent technique was used to investigate the potency of HNTs and NH(2)-MWCNTs in enhancing the transfection and expression efficiency of the DNA vaccine in Chinese hamster ovary (CHO) cells. Hamsters were immunised with two doses of vaccines HNT-pTARGET/lemA, NH(2)-MWCNTs-pTARGET/lemA, pTARGET/lemA, and empty pTARGET (control), and the efficacy was determined in terms of humoral immune response and protection against a lethal challenge. FINDINGS: rLemA DNA vaccines carried by NPs were able to transfect CHO cells effectively, inducing IgG immune response in hamsters (p < 0.05), and did not exhibit cytotoxic effects. Furthermore, 83.3% of the hamsters immunised with NH(2)-MWCNTs-pTARGET/lemA were protected against the lethal challenge (p < 0.01), and 66.7% of hamsters immunised with HNT-pTARGET/lemA survived (p < 0.05). MAIN CONCLUSIONS: NH(2)-MWCNTs and HNTs can act as antigen carriers for mammalian cells and are suitable for DNA nanovaccine delivery. Instituto Oswaldo Cruz, Ministério da Saúde 2020-04-17 /pmc/articles/PMC7164400/ /pubmed/32321154 http://dx.doi.org/10.1590/0074-02760190396 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Original Article Oliveira, Thaís Larré Bacelo, Kátia Leston Forster, Karine Maciel Ilha, Vinicius Rodrigues, Oscar Endrigo Hartwig, Daiane D DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection |
title | DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection |
title_full | DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection |
title_fullStr | DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection |
title_full_unstemmed | DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection |
title_short | DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection |
title_sort | dna nanovaccines prepared using lema antigen protect golden syrian hamsters against leptospira lethal infection |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164400/ https://www.ncbi.nlm.nih.gov/pubmed/32321154 http://dx.doi.org/10.1590/0074-02760190396 |
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