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An improved reverse genetics system for generation of bovine viral diarrhea virus as a BAC cDNA

Full length cDNA clones of bovine viral diarrhea virus (BVDV) with a low-copy number plasmid backbone have not proven to be stable when propagated in bacteria. To improve stability, pBAS, a bacterial artificial chromosome (BAC) plasmid was used to construct a full length cDNA clone of BVDV strain SD...

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Detalles Bibliográficos
Autores principales: Fan, Zhen-Chuan, Bird, R. Curtis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172198/
https://www.ncbi.nlm.nih.gov/pubmed/18374992
http://dx.doi.org/10.1016/j.jviromet.2008.01.011