Development of a SYBR green I-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine epidemic diarrhea virus and porcine circovirus 3

The development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescen...

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Detalles Bibliográficos
Autores principales: Han, Hao-Ying, Zheng, Hui-Hua, Zhao, Yu, Tian, Run-Bo, Xu, Peng-Li, Hou, Hua-Lin, Chen, Hong-Ying, Yang, Ming-Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172278/
https://www.ncbi.nlm.nih.gov/pubmed/30735700
http://dx.doi.org/10.1016/j.mcp.2019.02.002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172278/
https://www.ncbi.nlm.nih.gov/pubmed/30735700
http://dx.doi.org/10.1016/j.mcp.2019.02.002

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