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Expression in Escherichia coli and purification of biologically active L proteinase of foot-and-mouth disease virus

The foot-and-mouth disease virus (FMDV) Lb gene was cloned into bacterial expression vectors under the control of a T7 RNA polymerase promoter. The Lb protein was expressed in both an in vitro transcription-translation system and in Escherichia coli. In vitro expression of a construct containing the...

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Detalles Bibliográficos
Autores principales: Piccone, Maria E., Sira, Serge, Zellner, Marla, Grubman, Marvin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172946/
https://www.ncbi.nlm.nih.gov/pubmed/7785315
http://dx.doi.org/10.1016/0168-1702(94)00084-P