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Expression in Escherichia coli and purification of biologically active L proteinase of foot-and-mouth disease virus
The foot-and-mouth disease virus (FMDV) Lb gene was cloned into bacterial expression vectors under the control of a T7 RNA polymerase promoter. The Lb protein was expressed in both an in vitro transcription-translation system and in Escherichia coli. In vitro expression of a construct containing the...
Autores principales: | Piccone, Maria E., Sira, Serge, Zellner, Marla, Grubman, Marvin J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172946/ https://www.ncbi.nlm.nih.gov/pubmed/7785315 http://dx.doi.org/10.1016/0168-1702(94)00084-P |
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