Cargando…

Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice

Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted i...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Yu-Chao, He, Yong, Liu, Jian-Fa, Chen, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174000/
https://www.ncbi.nlm.nih.gov/pubmed/32315596
http://dx.doi.org/10.1051/parasite/2020021
_version_ 1783524551035977728
author Zhu, Yu-Chao
He, Yong
Liu, Jian-Fa
Chen, Jia
author_facet Zhu, Yu-Chao
He, Yong
Liu, Jian-Fa
Chen, Jia
author_sort Zhu, Yu-Chao
collection PubMed
description Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites.
format Online
Article
Text
id pubmed-7174000
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher EDP Sciences
record_format MEDLINE/PubMed
spelling pubmed-71740002020-04-22 Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice Zhu, Yu-Chao He, Yong Liu, Jian-Fa Chen, Jia Parasite Research Article Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites. EDP Sciences 2020-04-21 /pmc/articles/PMC7174000/ /pubmed/32315596 http://dx.doi.org/10.1051/parasite/2020021 Text en © Y.-C. Zhu et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhu, Yu-Chao
He, Yong
Liu, Jian-Fa
Chen, Jia
Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
title Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
title_full Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
title_fullStr Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
title_full_unstemmed Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
title_short Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
title_sort adjuvantic cytokine il-33 improves the protective immunity of cocktailed dna vaccine of rop5 and rop18 against toxoplasma gondii infection in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174000/
https://www.ncbi.nlm.nih.gov/pubmed/32315596
http://dx.doi.org/10.1051/parasite/2020021
work_keys_str_mv AT zhuyuchao adjuvanticcytokineil33improvestheprotectiveimmunityofcocktaileddnavaccineofrop5androp18againsttoxoplasmagondiiinfectioninmice
AT heyong adjuvanticcytokineil33improvestheprotectiveimmunityofcocktaileddnavaccineofrop5androp18againsttoxoplasmagondiiinfectioninmice
AT liujianfa adjuvanticcytokineil33improvestheprotectiveimmunityofcocktaileddnavaccineofrop5androp18againsttoxoplasmagondiiinfectioninmice
AT chenjia adjuvanticcytokineil33improvestheprotectiveimmunityofcocktaileddnavaccineofrop5androp18againsttoxoplasmagondiiinfectioninmice