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Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies

Enzymatic hydrolysis of soybean milk proteins with cysteine protease papain was performed in an advanced bioreactor, operated with batch mode. In soybean milk protein hydrolysis reaction, enzyme and substrate ratio and reaction temperature were varied, ranging from 0.029:100–0.457:100 and 30–60 °C,...

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Autores principales: Nath, Arijit, Kailo, Geremew Geidare, Mednyánszky, Zsuzsanna, Kiskó, Gabriella, Csehi, Barbara, Pásztorné-Huszár, Klára, Gerencsér-Berta, Renáta, Galambos, Ildikó, Pozsgai, Emília, Bánvölgyi, Szilvia, Vatai, Gyula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175099/
https://www.ncbi.nlm.nih.gov/pubmed/31905687
http://dx.doi.org/10.3390/bioengineering7010005
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author Nath, Arijit
Kailo, Geremew Geidare
Mednyánszky, Zsuzsanna
Kiskó, Gabriella
Csehi, Barbara
Pásztorné-Huszár, Klára
Gerencsér-Berta, Renáta
Galambos, Ildikó
Pozsgai, Emília
Bánvölgyi, Szilvia
Vatai, Gyula
author_facet Nath, Arijit
Kailo, Geremew Geidare
Mednyánszky, Zsuzsanna
Kiskó, Gabriella
Csehi, Barbara
Pásztorné-Huszár, Klára
Gerencsér-Berta, Renáta
Galambos, Ildikó
Pozsgai, Emília
Bánvölgyi, Szilvia
Vatai, Gyula
author_sort Nath, Arijit
collection PubMed
description Enzymatic hydrolysis of soybean milk proteins with cysteine protease papain was performed in an advanced bioreactor, operated with batch mode. In soybean milk protein hydrolysis reaction, enzyme and substrate ratio and reaction temperature were varied, ranging from 0.029:100–0.457:100 and 30–60 °C, respectively. The degree of hydrolysis of soybean milk proteins was increased with increase of enzyme and substrate (soybean milk protein) ratio. However, the degree of hydrolysis was increased due to change of reaction temperature from 30 °C to 60 °C with enzyme and substrate ratio 0.229:100 and was reduced when hydrolysis reaction was performed with enzyme and substrate ratio 0.11:100 at hydrolysis temperature 60 °C. Antioxidant capacity of enzyme-treated milk had a similar trend with degree of hydrolysis. In a later exercise, a membrane bioreactor was adopted for continuous production of antioxidant and antibacterial peptides from soybean milk. The membrane bioreactor was operated for 12 h with constant feeding. Ceramic-made tubular membrane with a pore size 20 nm was used. Application of static turbulence promoter in a membrane separation process was investigated and its positive effects, with respect to higher permeate flux and lower energy consumption in filtration process, were proven. Antioxidant capacity and antibacterial activity against Bacillus cereus of enzyme-hydrolyzed milk and permeate from membrane were confirmed.
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spelling pubmed-71750992020-04-28 Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies Nath, Arijit Kailo, Geremew Geidare Mednyánszky, Zsuzsanna Kiskó, Gabriella Csehi, Barbara Pásztorné-Huszár, Klára Gerencsér-Berta, Renáta Galambos, Ildikó Pozsgai, Emília Bánvölgyi, Szilvia Vatai, Gyula Bioengineering (Basel) Article Enzymatic hydrolysis of soybean milk proteins with cysteine protease papain was performed in an advanced bioreactor, operated with batch mode. In soybean milk protein hydrolysis reaction, enzyme and substrate ratio and reaction temperature were varied, ranging from 0.029:100–0.457:100 and 30–60 °C, respectively. The degree of hydrolysis of soybean milk proteins was increased with increase of enzyme and substrate (soybean milk protein) ratio. However, the degree of hydrolysis was increased due to change of reaction temperature from 30 °C to 60 °C with enzyme and substrate ratio 0.229:100 and was reduced when hydrolysis reaction was performed with enzyme and substrate ratio 0.11:100 at hydrolysis temperature 60 °C. Antioxidant capacity of enzyme-treated milk had a similar trend with degree of hydrolysis. In a later exercise, a membrane bioreactor was adopted for continuous production of antioxidant and antibacterial peptides from soybean milk. The membrane bioreactor was operated for 12 h with constant feeding. Ceramic-made tubular membrane with a pore size 20 nm was used. Application of static turbulence promoter in a membrane separation process was investigated and its positive effects, with respect to higher permeate flux and lower energy consumption in filtration process, were proven. Antioxidant capacity and antibacterial activity against Bacillus cereus of enzyme-hydrolyzed milk and permeate from membrane were confirmed. MDPI 2019-12-28 /pmc/articles/PMC7175099/ /pubmed/31905687 http://dx.doi.org/10.3390/bioengineering7010005 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nath, Arijit
Kailo, Geremew Geidare
Mednyánszky, Zsuzsanna
Kiskó, Gabriella
Csehi, Barbara
Pásztorné-Huszár, Klára
Gerencsér-Berta, Renáta
Galambos, Ildikó
Pozsgai, Emília
Bánvölgyi, Szilvia
Vatai, Gyula
Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
title Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
title_full Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
title_fullStr Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
title_full_unstemmed Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
title_short Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
title_sort antioxidant and antibacterial peptides from soybean milk through enzymatic- and membrane-based technologies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175099/
https://www.ncbi.nlm.nih.gov/pubmed/31905687
http://dx.doi.org/10.3390/bioengineering7010005
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