The Effects of Lactose Induction on a Plasmid-Free E. coli T7 Expression System

Recombinant production of pharmaceutical proteins like antigen binding fragments (Fabs) in the commonly-used production host Escherichia coli presents several challenges. The predominantly-used plasmid-based expression systems exhibit the drawback of either excessive plasmid amplification or plasmid...

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Detalles Bibliográficos
Autores principales: Hausjell, Johanna, Kutscha, Regina, Gesson, Jeannine D., Reinisch, Daniela, Spadiut, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175309/
https://www.ncbi.nlm.nih.gov/pubmed/31935883
http://dx.doi.org/10.3390/bioengineering7010008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175309/
https://www.ncbi.nlm.nih.gov/pubmed/31935883
http://dx.doi.org/10.3390/bioengineering7010008

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