Cargando…
The Effects of Lactose Induction on a Plasmid-Free E. coli T7 Expression System
Recombinant production of pharmaceutical proteins like antigen binding fragments (Fabs) in the commonly-used production host Escherichia coli presents several challenges. The predominantly-used plasmid-based expression systems exhibit the drawback of either excessive plasmid amplification or plasmid...
Autores principales: | Hausjell, Johanna, Kutscha, Regina, Gesson, Jeannine D., Reinisch, Daniela, Spadiut, Oliver |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175309/ https://www.ncbi.nlm.nih.gov/pubmed/31935883 http://dx.doi.org/10.3390/bioengineering7010008 |
Ejemplares similares
-
E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)
por: Hausjell, Johanna, et al.
Publicado: (2018) -
The E. coli pET expression system revisited—mechanistic correlation between glucose and lactose uptake
por: Wurm, David Johannes, et al.
Publicado: (2016) -
Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains
por: Wurm, David J., et al.
Publicado: (2017) -
Recombinant production of eukaryotic cytochrome P450s in microbial cell factories
por: Hausjell, Johanna, et al.
Publicado: (2018) -
The Lazarus Escherichia coli Effect: Recovery of Productivity on Glycerol/Lactose Mixed Feed in Continuous Biomanufacturing
por: Kittler, Stefan, et al.
Publicado: (2020)