Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in Pichia pastoris for Solid-State NMR

Solid-state nuclear magnetic resonance (SSNMR) is a powerful biophysical technique for studies of membrane proteins; it requires the incorporation of isotopic labels into the sample. This is usually accomplished through over-expression of the protein of interest in a prokaryotic or eukaryotic host i...

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Detalles Bibliográficos
Autores principales: Munro, Rachel, de Vlugt, Jeffrey, Ladizhansky, Vladimir, Brown, Leonid S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175339/
https://www.ncbi.nlm.nih.gov/pubmed/32168846
http://dx.doi.org/10.3390/biom10030434

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175339/
https://www.ncbi.nlm.nih.gov/pubmed/32168846
http://dx.doi.org/10.3390/biom10030434

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