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Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei

We investigated whether sequential reprogramming via porcine induced pluripotent stem cells (piPSCs) or exposure to oocyte cytoplasm following nuclear transfer could generate nuclear transfer-derived ESCs (piPSCs-ntESCs). Nuclear transfer embryos were reconstructed with piPSCs possessing a ZsGreen f...

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Detalles Bibliográficos
Autores principales: HARAGUCHI, Seiki, DANG-NGUYEN, Thanh Quang, WELLS, David, FUCHIMOTO, Daiichiro, FUKUDA, Tomokazu, TOKUNAGA, Tomoyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175389/
https://www.ncbi.nlm.nih.gov/pubmed/31983707
http://dx.doi.org/10.1262/jrd.2019-137