Cargando…

Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides

Dietary trans fatty acids (TFAs) have been implicated in serious health risks, yet little is known about their cellular effects and metabolism. We aim to undertake an in vitro comparison of two representative TFAs (elaidate and vaccenate) to the best-characterized endogenous cis-unsaturated FA (olea...

Descripción completa

Detalles Bibliográficos
Autores principales: Sarnyai, Farkas, Somogyi, Anna, Gór-Nagy, Zsófia, Zámbó, Veronika, Szelényi, Péter, Mátyási, Judit, Simon-Szabó, Laura, Kereszturi, Éva, Tóth, Blanka, Csala, Miklós
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178055/
https://www.ncbi.nlm.nih.gov/pubmed/32283839
http://dx.doi.org/10.3390/ijms21072626
_version_ 1783525367104929792
author Sarnyai, Farkas
Somogyi, Anna
Gór-Nagy, Zsófia
Zámbó, Veronika
Szelényi, Péter
Mátyási, Judit
Simon-Szabó, Laura
Kereszturi, Éva
Tóth, Blanka
Csala, Miklós
author_facet Sarnyai, Farkas
Somogyi, Anna
Gór-Nagy, Zsófia
Zámbó, Veronika
Szelényi, Péter
Mátyási, Judit
Simon-Szabó, Laura
Kereszturi, Éva
Tóth, Blanka
Csala, Miklós
author_sort Sarnyai, Farkas
collection PubMed
description Dietary trans fatty acids (TFAs) have been implicated in serious health risks, yet little is known about their cellular effects and metabolism. We aim to undertake an in vitro comparison of two representative TFAs (elaidate and vaccenate) to the best-characterized endogenous cis-unsaturated FA (oleate). The present study addresses the possible protective action of TFAs on palmitate-treated RINm5F insulinoma cells with special regards to apoptosis, endoplasmic reticulum stress and the underlying ceramide and diglyceride (DG) accumulation. Both TFAs significantly improved cell viability and reduced apoptosis in palmitate-treated cells. They mildly attenuated palmitate-induced XBP-1 mRNA cleavage and phosphorylation of eukaryotic initiation factor 2α (eIF2α) and stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK), but they were markedly less potent than oleate. Accordingly, all the three unsaturated FAs markedly reduced cellular palmitate incorporation and prevented harmful ceramide and DG accumulation. However, more elaidate or vaccenate than oleate was inserted into ceramides and DGs. Our results revealed a protective effect of TFAs in short-term palmitate toxicity, yet they also provide important in vitro evidence and even a potential mechanism for unfavorable long-term health effects of TFAs compared to oleate.
format Online
Article
Text
id pubmed-7178055
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71780552020-04-28 Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides Sarnyai, Farkas Somogyi, Anna Gór-Nagy, Zsófia Zámbó, Veronika Szelényi, Péter Mátyási, Judit Simon-Szabó, Laura Kereszturi, Éva Tóth, Blanka Csala, Miklós Int J Mol Sci Article Dietary trans fatty acids (TFAs) have been implicated in serious health risks, yet little is known about their cellular effects and metabolism. We aim to undertake an in vitro comparison of two representative TFAs (elaidate and vaccenate) to the best-characterized endogenous cis-unsaturated FA (oleate). The present study addresses the possible protective action of TFAs on palmitate-treated RINm5F insulinoma cells with special regards to apoptosis, endoplasmic reticulum stress and the underlying ceramide and diglyceride (DG) accumulation. Both TFAs significantly improved cell viability and reduced apoptosis in palmitate-treated cells. They mildly attenuated palmitate-induced XBP-1 mRNA cleavage and phosphorylation of eukaryotic initiation factor 2α (eIF2α) and stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK), but they were markedly less potent than oleate. Accordingly, all the three unsaturated FAs markedly reduced cellular palmitate incorporation and prevented harmful ceramide and DG accumulation. However, more elaidate or vaccenate than oleate was inserted into ceramides and DGs. Our results revealed a protective effect of TFAs in short-term palmitate toxicity, yet they also provide important in vitro evidence and even a potential mechanism for unfavorable long-term health effects of TFAs compared to oleate. MDPI 2020-04-09 /pmc/articles/PMC7178055/ /pubmed/32283839 http://dx.doi.org/10.3390/ijms21072626 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sarnyai, Farkas
Somogyi, Anna
Gór-Nagy, Zsófia
Zámbó, Veronika
Szelényi, Péter
Mátyási, Judit
Simon-Szabó, Laura
Kereszturi, Éva
Tóth, Blanka
Csala, Miklós
Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
title Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
title_full Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
title_fullStr Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
title_full_unstemmed Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
title_short Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides
title_sort effect of cis- and trans-monounsaturated fatty acids on palmitate toxicity and on palmitate-induced accumulation of ceramides and diglycerides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178055/
https://www.ncbi.nlm.nih.gov/pubmed/32283839
http://dx.doi.org/10.3390/ijms21072626
work_keys_str_mv AT sarnyaifarkas effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT somogyianna effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT gornagyzsofia effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT zamboveronika effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT szelenyipeter effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT matyasijudit effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT simonszabolaura effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT kereszturieva effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT tothblanka effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides
AT csalamiklos effectofcisandtransmonounsaturatedfattyacidsonpalmitatetoxicityandonpalmitateinducedaccumulationofceramidesanddiglycerides