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One-Pot Production of RNA in High Yield and Purity Through Cleaving Tandem Transcripts

There is an increasing demand for efficient and robust production of short RNA molecules in both pharmaceutics and research. A standard method is in vitro transcription by T7 RNA polymerase. This method is sequence-dependent on efficiency and is limited to products longer than ~12 nucleotides. Addit...

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Detalles Bibliográficos
Autores principales: Feyrer, Hannes, Munteanu, Raluca, Baronti, Lorenzo, Petzold, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179201/
https://www.ncbi.nlm.nih.gov/pubmed/32143353
http://dx.doi.org/10.3390/molecules25051142