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One-Pot Production of RNA in High Yield and Purity Through Cleaving Tandem Transcripts
There is an increasing demand for efficient and robust production of short RNA molecules in both pharmaceutics and research. A standard method is in vitro transcription by T7 RNA polymerase. This method is sequence-dependent on efficiency and is limited to products longer than ~12 nucleotides. Addit...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179201/ https://www.ncbi.nlm.nih.gov/pubmed/32143353 http://dx.doi.org/10.3390/molecules25051142 |