Cargando…

One-Pot Production of RNA in High Yield and Purity Through Cleaving Tandem Transcripts

There is an increasing demand for efficient and robust production of short RNA molecules in both pharmaceutics and research. A standard method is in vitro transcription by T7 RNA polymerase. This method is sequence-dependent on efficiency and is limited to products longer than ~12 nucleotides. Addit...

Descripción completa

Detalles Bibliográficos
Autores principales:	Feyrer, Hannes, Munteanu, Raluca, Baronti, Lorenzo, Petzold, Katja
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179201/ https://www.ncbi.nlm.nih.gov/pubmed/32143353 http://dx.doi.org/10.3390/molecules25051142

Ejemplares similares

A robust and versatile method for production and purification of large-scale RNA samples for structural biology
por: Karlsson, Hampus, et al.
Publicado: (2020)

Aminoboranes via Tandem Iodination/Dehydroiodination for One-Pot Borylation
por: Ramachandran, P. Veeraraghavan, et al.
Publicado: (2022)

High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories
por: Summerauer, Andrea Maria, et al.
Publicado: (2018)

Enzymatic incorporation of an isotope-labeled adenine into RNA for the study of conformational dynamics by NMR
por: Feyrer, Hannes, et al.
Publicado: (2022)

One-Pot Tandem Hiyama Alkynylation/Cyclizations by Palladium(II) Acyclic Diaminocarbene (ADC) Complexes Yielding Biologically Relevant Benzofuran Scaffolds
por: Singh, Chandan, et al.
Publicado: (2018)

A one-pot multistep cyclization yielding thiadiazoloimidazole derivatives
por: Samanta, Debabrata, et al.
Publicado: (2014)

One-Pot Tandem Synthesis of 2-Arylquinazolines by a Multicomponent Cyclization Reaction
por: Ye, Leping, et al.
Publicado: (2013)

One-Pot Tandem Approach to Functionalized 3-Hydroxy-2-furanyl-acrylamides
por: Mupparapu, Nagaraju, et al.
Publicado: (2018)

One-Pot Tandem Alcoholysis-Hydrogenation of Polylactic Acid to 1,2-Propanediol
por: Xu, Jialin, et al.
Publicado: (2023)

Nitrogen‐Doped Carbon‐Assisted One‐pot Tandem Reaction for Vinyl Chloride Production via Ethylene Oxychlorination
por: Ma, Hongfei, et al.
Publicado: (2020)

A guide to large-scale RNA sample preparation
por: Baronti, Lorenzo, et al.
Publicado: (2018)

Purity and yield of melanoma exosomes are dependent on isolation method
por: Shu, Shin La, et al.
Publicado: (2019)

A One-Pot Tandem Strategy in Catalytic Asymmetric Vinylogous Aldol Reaction of Homoallylic Alcohols
por: Hou, Xufeng, et al.
Publicado: (2016)

Computational and experimental demonstrations of one-pot tandem catalysis for electrochemical carbon dioxide reduction to methane
por: Zhang, Haochen, et al.
Publicado: (2019)

High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs
por: MalagodaPathiranage, Kithmie, et al.
Publicado: (2023)

Pot economy and one-pot synthesis
por: Hayashi, Yujiro
Publicado: (2016)

One-Pot Enzymatic Production of Lignin-Composites
por: Ion, Sabina, et al.
Publicado: (2018)

Determinants of DNA yield and purity collected with buccal cell samples
por: van Wieren-de Wijer, D. B. M. A., et al.
Publicado: (2009)

Bioretrosynthesis of Functionalized N‐Heterocycles from Glucose via One‐Pot Tandem Collaborations of Designed Microbes
por: Feng, Jing, et al.
Publicado: (2020)

The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles
por: Cvjetkovic, Aleksander, et al.
Publicado: (2014)

Defined MSC exosome with high yield and purity to improve regenerative activity
por: Kim, Jun Yong, et al.
Publicado: (2021)

The Role Ionic Liquid [BMIM][PF(6)] in One-Pot Synthesis of Tetrahydropyran Rings through Tandem Barbier–Prins Reaction
por: Batista, Poliane K., et al.
Publicado: (2019)

Programing a cyanide-free transformation of aldehydes to nitriles and one-pot synthesis of amides through tandem chemo-enzymatic cascades
por: Zheng, Haoteng, et al.
Publicado: (2022)

Catalyst-free synthesis of tetrahydrodipyrazolopyridines via an one-pot tandem and green pseudo-six-component reaction in water
por: Keihanfar, Mina, et al.
Publicado: (2022)

Construction of N‐Boc‐2‐Alkylaminoquinazolin‐4(3H)‐Ones via a Three‐Component, One‐Pot Protocol Mediated by Copper(II) Chloride that Spares Enantiomeric Purity
por: Li, Xiaoyu, et al.
Publicado: (2021)

A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
por: Amicucci, Matthew J., et al.
Publicado: (2020)

Tandem one-pot synthesis of 2-arylcinnolin-6-one derivatives from arylhydrazonopropanals and acetoacetanilides using sustainable ultrasound and microwave platforms
por: Al-Matar, Hamad M., et al.
Publicado: (2018)

High-yield ‘one-pot’ biosynthesis of raspberry ketone, a high-value fine chemical
por: Moore, Simon J, et al.
Publicado: (2021)

One-Pot Extraction and Quantification Method for Bile Acids in the Rat Liver by Capillary Liquid Chromatography Tandem Mass Spectrometry
por: Asano, Tomomi, et al.
Publicado: (2021)

One-pot synthesis of chiral alcohols from alkynes by CF(3)SO(3)H/ruthenium tandem catalysis
por: Liu, Huan, et al.
Publicado: (2018)

Leveraging Metal and Ligand Reactive Sites for One Pot Reactions: Ligand‐Centered Borenium Ions for Tandem Catalysis with Palladium
por: Skaria, Manisha, et al.
Publicado: (2022)

One-pot synthesis of (−)-Ambrox
por: Yang, Shaoxiang, et al.
Publicado: (2016)

A transcriptional blueprint for a spiral-cleaving embryo
por: Chou, Hsien-Chao, et al.
Publicado: (2016)

Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery
por: Gheinani, Ali Hashemi, et al.
Publicado: (2018)

One-pot tandem cyclization of enantiopure asymmetric cis-2,5-disubstituted pyrrolidines: Facile access to chiral 10-heteroazatriquinanes
por: Wang, Ping-An, et al.
Publicado: (2013)

NaH Promoted One-Pot Tandem Reactions of 3-(1-Alkynyl) Chromones to Form 2-Nitrogen-Substituted Xanthones
por: Duan, Wen-Di, et al.
Publicado: (2020)

One‐Pot Cooperation of Single‐Atom Rh and Ru Solid Catalysts for a Selective Tandem Olefin Isomerization‐Hydrosilylation Process
por: Sarma, Bidyut B., et al.
Publicado: (2020)

One-pot three-component tandem annulation of 4-hydroxycoumarine with aldehyde and aromatic amines using graphene oxide as an efficient catalyst
por: Singha, Rabindranath, et al.
Publicado: (2021)

Fully scalable one-pot method for the production of phosphonic graphene derivatives
por: Żelechowska, Kamila, et al.
Publicado: (2017)

Development of One Pot Strategy for Hyper Production and In Vivo Evaluation of Lovastatin
por: Azeem, Muhammad, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_62rg4qqo2hm12vuegb1ffokuuh