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Reliable measurement of free Ca(2+) concentrations in the ER lumen using Mag-Fluo-4
Synthetic Ca(2+) indicators are widely used to report changes in free [Ca(2+)], usually in the cytosol but also within organelles. Mag-Fluo-4, loaded into the endoplasmic reticulum (ER) by incubating cells with Mag-Fluo-4 AM, has been used to measure changes in free [Ca(2+)] within the ER, where the...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181174/ https://www.ncbi.nlm.nih.gov/pubmed/32179239 http://dx.doi.org/10.1016/j.ceca.2020.102188 |