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Reliable measurement of free Ca(2+) concentrations in the ER lumen using Mag-Fluo-4

Synthetic Ca(2+) indicators are widely used to report changes in free [Ca(2+)], usually in the cytosol but also within organelles. Mag-Fluo-4, loaded into the endoplasmic reticulum (ER) by incubating cells with Mag-Fluo-4 AM, has been used to measure changes in free [Ca(2+)] within the ER, where the...

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Detalles Bibliográficos
Autores principales: Rossi, Ana M., Taylor, Colin W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181174/
https://www.ncbi.nlm.nih.gov/pubmed/32179239
http://dx.doi.org/10.1016/j.ceca.2020.102188