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Functional efficiency of PCR vectors in vitro and at the organism level

The functional efficiency of the expression cassettes integrated into a plasmid and a PCR- amplified fragment was comparatively analyzed after transient transfection in vitro or introduction into the developing embryo of Danio rerio. The cassettes contained the reporter genes, luciferase of Photinus...

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Detalles Bibliográficos
Autores principales: Safina, Dina R., Selina, Polina I., Roschina, Marina P., Karaseva, Maria A., Komissarov, Alexey A., Demidyuk, Ilya V., Sverdlov, Eugene D., Kostrov, Sergey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182179/
https://www.ncbi.nlm.nih.gov/pubmed/32330156
http://dx.doi.org/10.1371/journal.pone.0232045