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Keth-seq for transcriptome-wide RNA structure mapping
RNA secondary structure is critical to RNA regulation and function. We report a new N(3)-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N(3)-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182492/ https://www.ncbi.nlm.nih.gov/pubmed/32015521 http://dx.doi.org/10.1038/s41589-019-0459-3 |