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Keth-seq for transcriptome-wide RNA structure mapping
RNA secondary structure is critical to RNA regulation and function. We report a new N(3)-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N(3)-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182492/ https://www.ncbi.nlm.nih.gov/pubmed/32015521 http://dx.doi.org/10.1038/s41589-019-0459-3 |
| _version_ | 1783526247559593984 |
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| author | Weng, Xiaocheng Gong, Jing Chen, Yi Wu, Tong Wang, Fang Yang, Shixi Yuan, Yushu Luo, Guanzheng Chen, Kai Hu, Lulu Ma, Honghui Wang, Pingluan Zhang, Qiangfeng Cliff Zhou, Xiang He, Chuan |
| author_facet | Weng, Xiaocheng Gong, Jing Chen, Yi Wu, Tong Wang, Fang Yang, Shixi Yuan, Yushu Luo, Guanzheng Chen, Kai Hu, Lulu Ma, Honghui Wang, Pingluan Zhang, Qiangfeng Cliff Zhou, Xiang He, Chuan |
| author_sort | Weng, Xiaocheng |
| collection | PubMed |
| description | RNA secondary structure is critical to RNA regulation and function. We report a new N(3)-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N(3)-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping. |
| format | Online Article Text |
| id | pubmed-7182492 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-71824922020-08-03 Keth-seq for transcriptome-wide RNA structure mapping Weng, Xiaocheng Gong, Jing Chen, Yi Wu, Tong Wang, Fang Yang, Shixi Yuan, Yushu Luo, Guanzheng Chen, Kai Hu, Lulu Ma, Honghui Wang, Pingluan Zhang, Qiangfeng Cliff Zhou, Xiang He, Chuan Nat Chem Biol Article RNA secondary structure is critical to RNA regulation and function. We report a new N(3)-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N(3)-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping. 2020-02-03 2020-05 /pmc/articles/PMC7182492/ /pubmed/32015521 http://dx.doi.org/10.1038/s41589-019-0459-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Weng, Xiaocheng Gong, Jing Chen, Yi Wu, Tong Wang, Fang Yang, Shixi Yuan, Yushu Luo, Guanzheng Chen, Kai Hu, Lulu Ma, Honghui Wang, Pingluan Zhang, Qiangfeng Cliff Zhou, Xiang He, Chuan Keth-seq for transcriptome-wide RNA structure mapping |
| title | Keth-seq for transcriptome-wide RNA structure mapping |
| title_full | Keth-seq for transcriptome-wide RNA structure mapping |
| title_fullStr | Keth-seq for transcriptome-wide RNA structure mapping |
| title_full_unstemmed | Keth-seq for transcriptome-wide RNA structure mapping |
| title_short | Keth-seq for transcriptome-wide RNA structure mapping |
| title_sort | keth-seq for transcriptome-wide rna structure mapping |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182492/ https://www.ncbi.nlm.nih.gov/pubmed/32015521 http://dx.doi.org/10.1038/s41589-019-0459-3 |
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