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Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing

Recently, the unexpected presence of a viable unauthorized genetically modified bacterium in a commercialized food enzyme (protease) product originating from a microbial fermentation process has been notified at the European level (RASFF 2019.3332). This finding was made possible thanks to the use o...

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Autores principales: Fraiture, Marie-Alice, Bogaerts, Bert, Winand, Raf, Deckers, Marie, Papazova, Nina, Vanneste, Kevin, De Keersmaecker, Sigrid C. J., Roosens, Nancy H. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184583/
https://www.ncbi.nlm.nih.gov/pubmed/32341433
http://dx.doi.org/10.1038/s41598-020-63987-5
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author Fraiture, Marie-Alice
Bogaerts, Bert
Winand, Raf
Deckers, Marie
Papazova, Nina
Vanneste, Kevin
De Keersmaecker, Sigrid C. J.
Roosens, Nancy H. C.
author_facet Fraiture, Marie-Alice
Bogaerts, Bert
Winand, Raf
Deckers, Marie
Papazova, Nina
Vanneste, Kevin
De Keersmaecker, Sigrid C. J.
Roosens, Nancy H. C.
author_sort Fraiture, Marie-Alice
collection PubMed
description Recently, the unexpected presence of a viable unauthorized genetically modified bacterium in a commercialized food enzyme (protease) product originating from a microbial fermentation process has been notified at the European level (RASFF 2019.3332). This finding was made possible thanks to the use of the next-generation sequencing technology, as reported in this study. Whole-genome sequencing was used to characterize the genetic modification comprising a sequence from the pUB110 shuttle vector (GenBank: M19465.1), harbouring antimicrobial resistance genes conferring a resistance to kanamycine, neomycin and bleomycin, flanked on each side by a sequence coding for a protease (GenBank: WP_032874795.1). In addition, based on these data, two real-time PCR methods, that can be used by enforcement laboratories, specific to this unauthorized genetically modified bacterium were developed and validated. The present study emphasizes the key role that whole-genome sequencing can take for detection of unknown and unauthorized genetically modified microorganisms in commercialized microbial fermentation products intended for the food and feed chain. Moreover, current issues encountered by the Competent Authorities and enforcement laboratories with such unexpected contaminations and the importance of performing official controls were highlighted.
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spelling pubmed-71845832020-04-29 Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing Fraiture, Marie-Alice Bogaerts, Bert Winand, Raf Deckers, Marie Papazova, Nina Vanneste, Kevin De Keersmaecker, Sigrid C. J. Roosens, Nancy H. C. Sci Rep Article Recently, the unexpected presence of a viable unauthorized genetically modified bacterium in a commercialized food enzyme (protease) product originating from a microbial fermentation process has been notified at the European level (RASFF 2019.3332). This finding was made possible thanks to the use of the next-generation sequencing technology, as reported in this study. Whole-genome sequencing was used to characterize the genetic modification comprising a sequence from the pUB110 shuttle vector (GenBank: M19465.1), harbouring antimicrobial resistance genes conferring a resistance to kanamycine, neomycin and bleomycin, flanked on each side by a sequence coding for a protease (GenBank: WP_032874795.1). In addition, based on these data, two real-time PCR methods, that can be used by enforcement laboratories, specific to this unauthorized genetically modified bacterium were developed and validated. The present study emphasizes the key role that whole-genome sequencing can take for detection of unknown and unauthorized genetically modified microorganisms in commercialized microbial fermentation products intended for the food and feed chain. Moreover, current issues encountered by the Competent Authorities and enforcement laboratories with such unexpected contaminations and the importance of performing official controls were highlighted. Nature Publishing Group UK 2020-04-27 /pmc/articles/PMC7184583/ /pubmed/32341433 http://dx.doi.org/10.1038/s41598-020-63987-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fraiture, Marie-Alice
Bogaerts, Bert
Winand, Raf
Deckers, Marie
Papazova, Nina
Vanneste, Kevin
De Keersmaecker, Sigrid C. J.
Roosens, Nancy H. C.
Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
title Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
title_full Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
title_fullStr Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
title_full_unstemmed Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
title_short Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
title_sort identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184583/
https://www.ncbi.nlm.nih.gov/pubmed/32341433
http://dx.doi.org/10.1038/s41598-020-63987-5
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