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Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()
In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated prot...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184670/ https://www.ncbi.nlm.nih.gov/pubmed/32222540 http://dx.doi.org/10.1016/j.ab.2020.113668 |
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author | Arike, Liisa Hansson, Gunnar C. Recktenwald, Christian V. |
author_facet | Arike, Liisa Hansson, Gunnar C. Recktenwald, Christian V. |
author_sort | Arike, Liisa |
collection | PubMed |
description | In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated protein/peptide are often neglected. This article describes a workflow for the detection of the products of transglutaminase-catalyzed reactions. In particular, possible pitfalls and traps that can arise during the mass spectrometry-based identification of isopeptide cross-links are addressed and characterised on actual samples. |
format | Online Article Text |
id | pubmed-7184670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-71846702020-05-15 Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() Arike, Liisa Hansson, Gunnar C. Recktenwald, Christian V. Anal Biochem Article In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated protein/peptide are often neglected. This article describes a workflow for the detection of the products of transglutaminase-catalyzed reactions. In particular, possible pitfalls and traps that can arise during the mass spectrometry-based identification of isopeptide cross-links are addressed and characterised on actual samples. Elsevier 2020-05-15 /pmc/articles/PMC7184670/ /pubmed/32222540 http://dx.doi.org/10.1016/j.ab.2020.113668 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Arike, Liisa Hansson, Gunnar C. Recktenwald, Christian V. Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() |
title | Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() |
title_full | Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() |
title_fullStr | Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() |
title_full_unstemmed | Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() |
title_short | Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() |
title_sort | identifying transglutaminase reaction products via mass spectrometry as exemplified by the muc2 mucin - pitfalls and traps() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184670/ https://www.ncbi.nlm.nih.gov/pubmed/32222540 http://dx.doi.org/10.1016/j.ab.2020.113668 |
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