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Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()

In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated prot...

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Autores principales: Arike, Liisa, Hansson, Gunnar C., Recktenwald, Christian V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184670/
https://www.ncbi.nlm.nih.gov/pubmed/32222540
http://dx.doi.org/10.1016/j.ab.2020.113668
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author Arike, Liisa
Hansson, Gunnar C.
Recktenwald, Christian V.
author_facet Arike, Liisa
Hansson, Gunnar C.
Recktenwald, Christian V.
author_sort Arike, Liisa
collection PubMed
description In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated protein/peptide are often neglected. This article describes a workflow for the detection of the products of transglutaminase-catalyzed reactions. In particular, possible pitfalls and traps that can arise during the mass spectrometry-based identification of isopeptide cross-links are addressed and characterised on actual samples.
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spelling pubmed-71846702020-05-15 Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps() Arike, Liisa Hansson, Gunnar C. Recktenwald, Christian V. Anal Biochem Article In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated protein/peptide are often neglected. This article describes a workflow for the detection of the products of transglutaminase-catalyzed reactions. In particular, possible pitfalls and traps that can arise during the mass spectrometry-based identification of isopeptide cross-links are addressed and characterised on actual samples. Elsevier 2020-05-15 /pmc/articles/PMC7184670/ /pubmed/32222540 http://dx.doi.org/10.1016/j.ab.2020.113668 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arike, Liisa
Hansson, Gunnar C.
Recktenwald, Christian V.
Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()
title Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()
title_full Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()
title_fullStr Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()
title_full_unstemmed Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()
title_short Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and traps()
title_sort identifying transglutaminase reaction products via mass spectrometry as exemplified by the muc2 mucin - pitfalls and traps()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184670/
https://www.ncbi.nlm.nih.gov/pubmed/32222540
http://dx.doi.org/10.1016/j.ab.2020.113668
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