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Preparative and Kinetic Analysis of β‐1,4‐ and β‐1,3‐Glucan Phosphorylases Informs Access to Human Milk Oligosaccharide Fragments and Analogues Thereof

The enzymatic synthesis of oligosaccharides depends on the availability of suitable enzymes, which remains a limitation. Without recourse to enzyme engineering or evolution approaches, herein we demonstrate the ability of wild‐type cellodextrin phosphorylase (CDP: β‐1,4‐glucan linkage‐dependent) and...

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Detalles Bibliográficos
Autores principales: Singh, Ravindra Pal, Pergolizzi, Giulia, Nepogodiev, Sergey A., de Andrade, Peterson, Kuhaudomlarp, Sakonwan, Field, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187349/
https://www.ncbi.nlm.nih.gov/pubmed/31657512
http://dx.doi.org/10.1002/cbic.201900440