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Preparative and Kinetic Analysis of β‐1,4‐ and β‐1,3‐Glucan Phosphorylases Informs Access to Human Milk Oligosaccharide Fragments and Analogues Thereof
The enzymatic synthesis of oligosaccharides depends on the availability of suitable enzymes, which remains a limitation. Without recourse to enzyme engineering or evolution approaches, herein we demonstrate the ability of wild‐type cellodextrin phosphorylase (CDP: β‐1,4‐glucan linkage‐dependent) and...
Autores principales: | Singh, Ravindra Pal, Pergolizzi, Giulia, Nepogodiev, Sergey A., de Andrade, Peterson, Kuhaudomlarp, Sakonwan, Field, Robert A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187349/ https://www.ncbi.nlm.nih.gov/pubmed/31657512 http://dx.doi.org/10.1002/cbic.201900440 |
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