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Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin

The lack of tools to reliably detect RanBP9 in vivo has significantly hampered progress in understanding the biological functions of this scaffold protein. We report here the generation of a novel mouse strain, RanBP9-TT, in which the endogenous protein is fused with a double (V5-HA) epitope tag at...

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Detalles Bibliográficos
Autores principales: Soliman, Shimaa H. A., Stark, Aaron E., Gardner, Miranda L., Harshman, Sean W., Breece, Chelssie C., Amari, Foued, Orlacchio, Arturo, Chen, Min, Tessari, Anna, Martin, Jennifer A., Visone, Rosa, Freitas, Michael A., La Perle, Krista M. D., Palmieri, Dario, Coppola, Vincenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188826/
https://www.ncbi.nlm.nih.gov/pubmed/32346083
http://dx.doi.org/10.1038/s41598-020-64047-8