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Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin
The lack of tools to reliably detect RanBP9 in vivo has significantly hampered progress in understanding the biological functions of this scaffold protein. We report here the generation of a novel mouse strain, RanBP9-TT, in which the endogenous protein is fused with a double (V5-HA) epitope tag at...
Autores principales: | Soliman, Shimaa H. A., Stark, Aaron E., Gardner, Miranda L., Harshman, Sean W., Breece, Chelssie C., Amari, Foued, Orlacchio, Arturo, Chen, Min, Tessari, Anna, Martin, Jennifer A., Visone, Rosa, Freitas, Michael A., La Perle, Krista M. D., Palmieri, Dario, Coppola, Vincenzo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188826/ https://www.ncbi.nlm.nih.gov/pubmed/32346083 http://dx.doi.org/10.1038/s41598-020-64047-8 |
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