A Fast and Accurate Method to Identify and Quantify Enzymes in Brush-Border Membranes: In Situ Hydrolysis Followed by Nano LC-MS/MS

A simple method for the identification of brush-border membrane α-glucosidases is described. The proteins were first solubilized and separated in a gel under native, non-denaturing, conditions. The gel was then incubated in substrate solutions (maltose or sucrose), and the product (glucose) exposed...

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Detalles Bibliográficos
Autores principales: Brun, Antonio, Magallanes, Melisa E., Martínez del Rio, Carlos, Barrett-Wilt, Gregory A., Karasov, William H., Caviedes-Vidal, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189658/
https://www.ncbi.nlm.nih.gov/pubmed/32050538
http://dx.doi.org/10.3390/mps3010015

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189658/
https://www.ncbi.nlm.nih.gov/pubmed/32050538
http://dx.doi.org/10.3390/mps3010015

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