Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition

• Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins.

Detalles Bibliográficos
Autores principales: Karimi Baba ahmadi, Mohammad, Mohammadi, Seyed Abolghasem, Makvandi, Manoochehr, Mamoueie, Morteza, Rahmati, Mohammad, Wood, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189850/
https://www.ncbi.nlm.nih.gov/pubmed/32360597
http://dx.doi.org/10.1016/j.pep.2020.105650

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189850/
https://www.ncbi.nlm.nih.gov/pubmed/32360597
http://dx.doi.org/10.1016/j.pep.2020.105650

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