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Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition

• Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins.

Detalles Bibliográficos
Autores principales: Karimi Baba ahmadi, Mohammad, Mohammadi, Seyed Abolghasem, Makvandi, Manoochehr, Mamoueie, Morteza, Rahmati, Mohammad, Wood, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189850/
https://www.ncbi.nlm.nih.gov/pubmed/32360597
http://dx.doi.org/10.1016/j.pep.2020.105650
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author Karimi Baba ahmadi, Mohammad
Mohammadi, Seyed Abolghasem
Makvandi, Manoochehr
Mamoueie, Morteza
Rahmati, Mohammad
Wood, David
author_facet Karimi Baba ahmadi, Mohammad
Mohammadi, Seyed Abolghasem
Makvandi, Manoochehr
Mamoueie, Morteza
Rahmati, Mohammad
Wood, David
author_sort Karimi Baba ahmadi, Mohammad
collection PubMed
description • Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins.
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spelling pubmed-71898502020-04-29 Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition Karimi Baba ahmadi, Mohammad Mohammadi, Seyed Abolghasem Makvandi, Manoochehr Mamoueie, Morteza Rahmati, Mohammad Wood, David Protein Expr Purif Article • Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins. Published by Elsevier Inc. 2020-10 2020-04-29 /pmc/articles/PMC7189850/ /pubmed/32360597 http://dx.doi.org/10.1016/j.pep.2020.105650 Text en © 2020 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Karimi Baba ahmadi, Mohammad
Mohammadi, Seyed Abolghasem
Makvandi, Manoochehr
Mamoueie, Morteza
Rahmati, Mohammad
Wood, David
Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
title Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
title_full Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
title_fullStr Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
title_full_unstemmed Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
title_short Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
title_sort column-free purification and coating of spycatcher protein on elisa wells generates universal solid support for capturing of spytag-fusion protein from the non-purified condition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189850/
https://www.ncbi.nlm.nih.gov/pubmed/32360597
http://dx.doi.org/10.1016/j.pep.2020.105650
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