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Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
• Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins.
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189850/ https://www.ncbi.nlm.nih.gov/pubmed/32360597 http://dx.doi.org/10.1016/j.pep.2020.105650 |
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author | Karimi Baba ahmadi, Mohammad Mohammadi, Seyed Abolghasem Makvandi, Manoochehr Mamoueie, Morteza Rahmati, Mohammad Wood, David |
author_facet | Karimi Baba ahmadi, Mohammad Mohammadi, Seyed Abolghasem Makvandi, Manoochehr Mamoueie, Morteza Rahmati, Mohammad Wood, David |
author_sort | Karimi Baba ahmadi, Mohammad |
collection | PubMed |
description | • Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins. |
format | Online Article Text |
id | pubmed-7189850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71898502020-04-29 Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition Karimi Baba ahmadi, Mohammad Mohammadi, Seyed Abolghasem Makvandi, Manoochehr Mamoueie, Morteza Rahmati, Mohammad Wood, David Protein Expr Purif Article • Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins. Published by Elsevier Inc. 2020-10 2020-04-29 /pmc/articles/PMC7189850/ /pubmed/32360597 http://dx.doi.org/10.1016/j.pep.2020.105650 Text en © 2020 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Karimi Baba ahmadi, Mohammad Mohammadi, Seyed Abolghasem Makvandi, Manoochehr Mamoueie, Morteza Rahmati, Mohammad Wood, David Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition |
title | Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition |
title_full | Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition |
title_fullStr | Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition |
title_full_unstemmed | Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition |
title_short | Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition |
title_sort | column-free purification and coating of spycatcher protein on elisa wells generates universal solid support for capturing of spytag-fusion protein from the non-purified condition |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189850/ https://www.ncbi.nlm.nih.gov/pubmed/32360597 http://dx.doi.org/10.1016/j.pep.2020.105650 |
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