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Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-fusion protein from the non-purified condition
• Spy Tag-Protein covalent interaction is rapid and specific method for protein immobilization. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins.
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Published by Elsevier Inc.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189850/ https://www.ncbi.nlm.nih.gov/pubmed/32360597 http://dx.doi.org/10.1016/j.pep.2020.105650 |