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An artificial chromosome ylAC enables efficient assembly of multiple genes in Yarrowia lipolytica for biomanufacturing

The efficient use of the yeast Yarrowia lipolytica as a cell factory is hampered by the lack of powerful genetic engineering tools dedicated for the assembly of large DNA fragments and the robust expression of multiple genes. Here we describe the design and construction of artificial chromosomes (yl...

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Detalles Bibliográficos
Autores principales: Guo, Zhong-peng, Borsenberger, Vinciane, Croux, Christian, Duquesne, Sophie, Truan, Gilles, Marty, Alain, Bordes, Florence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7190667/
https://www.ncbi.nlm.nih.gov/pubmed/32350406
http://dx.doi.org/10.1038/s42003-020-0936-y