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Expression of Melittin in Fusion with GST in Escherichia coli and Its Purification as a Pure Peptide with Good Bacteriostatic Efficacy

[Image: see text] The expression and purification of melittin (MET) in microbials are difficult because of its antibacterial activities. In this work, MET was fused with a glutathione-S-transferase (GST) tag and expressed in Escherichia coli to overcome its lethality to host cells. The fusion protei...

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Detalles Bibliográficos
Autores principales: Zhou, Lixian, Liu, Zhiyong, Xu, Guanyu, Li, Lihong, Xuan, Kaiang, Xu, Yan, Zhang, Rongzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191569/
https://www.ncbi.nlm.nih.gov/pubmed/32363276
http://dx.doi.org/10.1021/acsomega.0c00085