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Expression of Melittin in Fusion with GST in Escherichia coli and Its Purification as a Pure Peptide with Good Bacteriostatic Efficacy
[Image: see text] The expression and purification of melittin (MET) in microbials are difficult because of its antibacterial activities. In this work, MET was fused with a glutathione-S-transferase (GST) tag and expressed in Escherichia coli to overcome its lethality to host cells. The fusion protei...
Autores principales: | Zhou, Lixian, Liu, Zhiyong, Xu, Guanyu, Li, Lihong, Xuan, Kaiang, Xu, Yan, Zhang, Rongzhen |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191569/ https://www.ncbi.nlm.nih.gov/pubmed/32363276 http://dx.doi.org/10.1021/acsomega.0c00085 |
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