Cargando…

A novel NGS library preparation method to characterize native termini of fragmented DNA

Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standar...

Descripción completa

Detalles Bibliográficos
Autores principales:	Harkins, Kelly M, Schaefer, Nathan K, Troll, Christopher J, Rao, Varsha, Kapp, Joshua, Naughton, Colin, Shapiro, Beth, Green, Richard E
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192605/ https://www.ncbi.nlm.nih.gov/pubmed/32112100 http://dx.doi.org/10.1093/nar/gkaa128

Ejemplares similares

A ligation-based single-stranded library preparation method to analyze cell-free DNA and synthetic oligos
por: Troll, Christopher J., et al.
Publicado: (2019)

A new method to prevent carry-over contaminations in two-step PCR NGS library preparations
por: Seitz, Volkhard, et al.
Publicado: (2015)

RepARK—de novo creation of repeat libraries from whole-genome NGS reads
por: Koch, Philipp, et al.
Publicado: (2014)

Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli
por: Nakashima, Nobutaka, et al.
Publicado: (2006)

A method to generate capture baits for targeted sequencing
por: Sundararaman, Balaji, et al.
Publicado: (2023)

An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments
por: Heyer, Erin E., et al.
Publicado: (2015)

Accurate SNP and mutation detection by targeted custom microarray-based genomic enrichment of short-fragment sequencing libraries
por: Mokry, Michal, et al.
Publicado: (2010)

Prioritizing and selecting likely novel miRNAs from NGS data
por: Backes, Christina, et al.
Publicado: (2016)

Count ratio model reveals bias affecting NGS fold changes
por: Erhard, Florian, et al.
Publicado: (2015)

Development of a high-resolution NGS-based HLA-typing and analysis pipeline
por: Wittig, Michael, et al.
Publicado: (2015)

Synthesis and cell-free cloning of DNA libraries using programmable microfluidics
por: Yehezkel, Tuval Ben, et al.
Publicado: (2016)

Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination
por: Van Nieuwerburgh, Filip, et al.
Publicado: (2012)

An efficient and sensitive method for preparing cDNA libraries from scarce biological samples
por: Sterling, Catherine H., et al.
Publicado: (2015)

NGS-based identification and tracing of microsatellite instability from minute amounts DNA using inter-Alu-PCR
por: Yu, Fangyan, et al.
Publicado: (2020)

Identification of soluble protein fragments by gene fragmentation and genetic selection
por: Dyson, Michael R., et al.
Publicado: (2008)

Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
por: Miura, Fumihito, et al.
Publicado: (2018)

Single-read tRNA-seq analysis reveals coordination of tRNA modification and aminoacylation and fragmentation
por: Hernandez-Alias, Xavier, et al.
Publicado: (2022)

Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase
por: Gansauge, Marie-Theres, et al.
Publicado: (2017)

A low-bias and sensitive small RNA library preparation method using randomized splint ligation
por: Maguire, Sean, et al.
Publicado: (2020)

Extensive chromatin fragmentation improves enrichment of protein binding sites in chromatin immunoprecipitation experiments
por: Fan, Xiaochun, et al.
Publicado: (2008)

SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
por: Raine, Amanda, et al.
Publicado: (2017)

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineering
por: Rivero-Müller, Adolfo, et al.
Publicado: (2007)

Targeting individual cells by barcode in pooled sequence libraries
por: Ranu, Navpreet, et al.
Publicado: (2019)

Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
por: Yang, Fang, et al.
Publicado: (2018)

The use of multiple displacement amplification to amplify complex DNA libraries
por: Fullwood, Melissa J., et al.
Publicado: (2008)

Selection of functional human antibodies from retroviral display libraries
por: Urban, Johannes H., et al.
Publicado: (2005)

Integrase-directed recovery of functional genes from genomic libraries
por: Rowe-Magnus, Dean A.
Publicado: (2009)

A transposase strategy for creating libraries of circularly permuted proteins
por: Mehta, Manan M., et al.
Publicado: (2012)

Phage display peptide libraries: deviations from randomness and correctives
por: Ryvkin, Arie, et al.
Publicado: (2018)

Flexible CRISPR library construction using parallel oligonucleotide retrieval
por: Read, Abigail, et al.
Publicado: (2017)

Extensive libraries of gene truncation variants generated by in vitro transposition
por: Morelli, Aleardo, et al.
Publicado: (2017)

Targeted insertional mutagenesis libraries for deep domain insertion profiling
por: Coyote-Maestas, Willow, et al.
Publicado: (2020)

Decoding the epitranscriptional landscape from native RNA sequences
por: Jenjaroenpun, Piroon, et al.
Publicado: (2020)

Bicistronic DNA display for in vitro selection of Fab fragments
por: Sumida, Takeshi, et al.
Publicado: (2009)

Promoter library designed for fine-tuned gene expression in Pichia pastoris
por: Hartner, Franz S., et al.
Publicado: (2008)

Covalent antibody display—an in vitro antibody-DNA library selection system
por: Reiersen, Herald, et al.
Publicado: (2005)

An on-bead tailing/ligation approach for sequencing resin-bound RNA libraries
por: Wiesmayr, Anna, et al.
Publicado: (2012)

Blocking of targeted microRNAs from next-generation sequencing libraries
por: Roberts, Brian S., et al.
Publicado: (2015)

A platform for functional assessment of large variant libraries in mammalian cells
por: Matreyek, Kenneth A., et al.
Publicado: (2017)

Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery
por: Xu, Peng, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_demm6st2o6do7t6ud5llr8ir4h