A Tandem Guide RNA-Based Strategy for Efficient CRISPR Gene Editing of Cell Populations with Low Heterogeneity of Edited Alleles

CRISPR/Cas9–based gene knockouts (KOs) enable precise perturbation of target gene function in human cells, which is ideally assessed in an unbiased fashion by molecular omics readouts. Typically, this requires the lengthy process of isolating KO subclones. We show here that KO subclones are phenotyp...

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Detalles Bibliográficos
Autores principales: Joberty, Gérard, Fälth-Savitski, Maria, Paulmann, Marcel, Bösche, Markus, Doce, Carola, Cheng, Aaron T, Drewes, Gerard, Grandi, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194318/
https://www.ncbi.nlm.nih.gov/pubmed/32315231
http://dx.doi.org/10.1089/crispr.2019.0064

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194318/
https://www.ncbi.nlm.nih.gov/pubmed/32315231
http://dx.doi.org/10.1089/crispr.2019.0064

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