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Selection and exploitation of prevalent, tandemly repeated genomic targets for improved real-time PCR-based detection of Wuchereria bancrofti and Plasmodium falciparum in mosquitoes

BACKGROUND: Optimization of polymerase chain reaction (PCR)-based diagnostics requires the careful selection of molecular targets that are both highly repetitive and pathogen-specific. Advances in both next-generation sequencing (NGS) technologies and bioinformatics-based analysis tools are facilita...

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Detalles Bibliográficos
Autores principales: Zulch, Michael F., Pilotte, Nils, Grant, Jessica R., Minetti, Corrado, Reimer, Lisa J., Williams, Steven A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194414/
https://www.ncbi.nlm.nih.gov/pubmed/32357154
http://dx.doi.org/10.1371/journal.pone.0232325