Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies

We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5′HVR ant...

Descripción completa

Detalles Bibliográficos
Autores principales: Cubillos-Zapata, Carolina, Angulo, Iván, Almanza, Horacio, Borrego, Belén, Zamora-Ceballos, María, Castón, José R., Mena, Ignacio, Blanco, Esther, Bárcena, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195702/
https://www.ncbi.nlm.nih.gov/pubmed/32357948
http://dx.doi.org/10.1186/s13567-020-00785-x
_version_ 1783528590317453312
author Cubillos-Zapata, Carolina
Angulo, Iván
Almanza, Horacio
Borrego, Belén
Zamora-Ceballos, María
Castón, José R.
Mena, Ignacio
Blanco, Esther
Bárcena, Juan
author_facet Cubillos-Zapata, Carolina
Angulo, Iván
Almanza, Horacio
Borrego, Belén
Zamora-Ceballos, María
Castón, José R.
Mena, Ignacio
Blanco, Esther
Bárcena, Juan
author_sort Cubillos-Zapata, Carolina
collection PubMed
description We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5′HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved and recognized by non-neutralizing MAbs; and a second epitope spanning amino acids 445-451 (ITTANQY), highly variable and recognized by neutralizing MAbs. These antibodies might be valuable for diagnostic applications, as well as for further research in different aspects of the biology of FCV.
format Online
Article
Text
id pubmed-7195702
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-71957022020-05-06 Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies Cubillos-Zapata, Carolina Angulo, Iván Almanza, Horacio Borrego, Belén Zamora-Ceballos, María Castón, José R. Mena, Ignacio Blanco, Esther Bárcena, Juan Vet Res Short Report We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5′HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved and recognized by non-neutralizing MAbs; and a second epitope spanning amino acids 445-451 (ITTANQY), highly variable and recognized by neutralizing MAbs. These antibodies might be valuable for diagnostic applications, as well as for further research in different aspects of the biology of FCV. BioMed Central 2020-05-01 2020 /pmc/articles/PMC7195702/ /pubmed/32357948 http://dx.doi.org/10.1186/s13567-020-00785-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Cubillos-Zapata, Carolina
Angulo, Iván
Almanza, Horacio
Borrego, Belén
Zamora-Ceballos, María
Castón, José R.
Mena, Ignacio
Blanco, Esther
Bárcena, Juan
Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies
title Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies
title_full Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies
title_fullStr Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies
title_full_unstemmed Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies
title_short Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies
title_sort precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195702/
https://www.ncbi.nlm.nih.gov/pubmed/32357948
http://dx.doi.org/10.1186/s13567-020-00785-x
work_keys_str_mv AT cubilloszapatacarolina preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT anguloivan preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT almanzahoracio preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT borregobelen preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT zamoraceballosmaria preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT castonjoser preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT menaignacio preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT blancoesther preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies
AT barcenajuan preciselocationoflinearepitopesonthecapsidsurfaceoffelinecalicivirusrecognizedbyneutralizingandnonneutralizingmonoclonalantibodies

Ejemplares similares