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Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia

BACKGROUND: We used bioinformatic tools to dichotomize 157 non-M3 AML patients from the TCGA dataset based on the presence or absence of TP53 mutations, and screened out a key gene related to TP53 mutation for future analysis. METHODS: DEGs were analyzed by R package “DESeq2” and then run GSEA, GO e...

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Autores principales: Gu, Siyu, Zi, Jie, Han, Qi, Song, Chunhua, Ge, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197135/
https://www.ncbi.nlm.nih.gov/pubmed/32390761
http://dx.doi.org/10.1186/s12935-020-01213-y
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author Gu, Siyu
Zi, Jie
Han, Qi
Song, Chunhua
Ge, Zheng
author_facet Gu, Siyu
Zi, Jie
Han, Qi
Song, Chunhua
Ge, Zheng
author_sort Gu, Siyu
collection PubMed
description BACKGROUND: We used bioinformatic tools to dichotomize 157 non-M3 AML patients from the TCGA dataset based on the presence or absence of TP53 mutations, and screened out a key gene related to TP53 mutation for future analysis. METHODS: DEGs were analyzed by R package “DESeq2” and then run GSEA, GO enrichment, KEGG pathway and PPI network. Hub genes were selected out according to MCC. Log-rank (Mantel–Cox) test was used for survival analysis. Mann–Whitney U’s nonparametric t test and Fisher’s exact test was used for continuous and categorical variables respectively. p value< 0.05 was considered to be statistical significance. RESULTS: TNFRSF4 was final screened out as a key gene. Besides TP53 mutation (p = 0.0118), high TNFRSF4 was also associated with FLT3 mutation (p = 0.0102) and NPM1 mutation (p = 0.0024). Elevated TNFRSF4 was significantly related with intermediate (p = 0.0004) and poor (p = 0.0011) risk stratification as well as relapse statute (p = 0.0099). Patients with elevated TNFRSF4 expression had significantly shorter overall survival (median survival: 2.35 months vs. 21 months, p < 0.0001). Based on our clinical center data, TNFRSF4 expression was significantly higher in non-M3 AML patients than HDs (p = 0.0377) and MDS patients (EB-1, 2; p = 0.0017). CONCLUSIONS: Elevated TNFRSF4 expression was associated with TP53, FLT3 and NPM1 mutation as well as poor clinical outcome. TNFRSF4 expression was significantly higher in non-M3 AML patients than HDs and MDS (EB-1, 2) patients. TNFRSF4 is need for future functional and mechanistic studies to investigate the role in non-M3 AML.
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spelling pubmed-71971352020-05-08 Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia Gu, Siyu Zi, Jie Han, Qi Song, Chunhua Ge, Zheng Cancer Cell Int Primary Research BACKGROUND: We used bioinformatic tools to dichotomize 157 non-M3 AML patients from the TCGA dataset based on the presence or absence of TP53 mutations, and screened out a key gene related to TP53 mutation for future analysis. METHODS: DEGs were analyzed by R package “DESeq2” and then run GSEA, GO enrichment, KEGG pathway and PPI network. Hub genes were selected out according to MCC. Log-rank (Mantel–Cox) test was used for survival analysis. Mann–Whitney U’s nonparametric t test and Fisher’s exact test was used for continuous and categorical variables respectively. p value< 0.05 was considered to be statistical significance. RESULTS: TNFRSF4 was final screened out as a key gene. Besides TP53 mutation (p = 0.0118), high TNFRSF4 was also associated with FLT3 mutation (p = 0.0102) and NPM1 mutation (p = 0.0024). Elevated TNFRSF4 was significantly related with intermediate (p = 0.0004) and poor (p = 0.0011) risk stratification as well as relapse statute (p = 0.0099). Patients with elevated TNFRSF4 expression had significantly shorter overall survival (median survival: 2.35 months vs. 21 months, p < 0.0001). Based on our clinical center data, TNFRSF4 expression was significantly higher in non-M3 AML patients than HDs (p = 0.0377) and MDS patients (EB-1, 2; p = 0.0017). CONCLUSIONS: Elevated TNFRSF4 expression was associated with TP53, FLT3 and NPM1 mutation as well as poor clinical outcome. TNFRSF4 expression was significantly higher in non-M3 AML patients than HDs and MDS (EB-1, 2) patients. TNFRSF4 is need for future functional and mechanistic studies to investigate the role in non-M3 AML. BioMed Central 2020-05-04 /pmc/articles/PMC7197135/ /pubmed/32390761 http://dx.doi.org/10.1186/s12935-020-01213-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Gu, Siyu
Zi, Jie
Han, Qi
Song, Chunhua
Ge, Zheng
Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia
title Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia
title_full Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia
title_fullStr Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia
title_full_unstemmed Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia
title_short Elevated TNFRSF4 gene expression is a predictor of poor prognosis in non-M3 acute myeloid leukemia
title_sort elevated tnfrsf4 gene expression is a predictor of poor prognosis in non-m3 acute myeloid leukemia
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197135/
https://www.ncbi.nlm.nih.gov/pubmed/32390761
http://dx.doi.org/10.1186/s12935-020-01213-y
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