Cargando…

Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis

Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived...

Descripción completa

Detalles Bibliográficos
Autores principales: Osborn, Daniel P. S., Li, Kuoyu, Cutty, Stephen J., Nelson, Andrew C., Wardle, Fiona C., Hinits, Yaniv, Hughes, Simon M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197714/
https://www.ncbi.nlm.nih.gov/pubmed/32345657
http://dx.doi.org/10.1242/dev.184689
_version_ 1783528873506373632
author Osborn, Daniel P. S.
Li, Kuoyu
Cutty, Stephen J.
Nelson, Andrew C.
Wardle, Fiona C.
Hinits, Yaniv
Hughes, Simon M.
author_facet Osborn, Daniel P. S.
Li, Kuoyu
Cutty, Stephen J.
Nelson, Andrew C.
Wardle, Fiona C.
Hinits, Yaniv
Hughes, Simon M.
author_sort Osborn, Daniel P. S.
collection PubMed
description Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived Fgf signals act through Tbx16 and Tbxta to induce slow and fast trunk muscle precursors at distinct dorsoventral positions. Tbx16 binds to and directly activates the myf5 and myod genes, which are required for commitment to myogenesis. Tbx16 activity depends on Fgf signalling from the organiser. In contrast, Tbxta is not required for myf5 expression, but binds a specific site upstream of myod that is not bound by Tbx16 and drives (dependent on Fgf signals) myod expression in adaxial slow precursors, thereby initiating trunk myogenesis. After gastrulation, when similar muscle cell populations in the post-anal tail are generated from tailbud, declining Fgf signalling is less effective at initiating adaxial myogenesis, which is instead initiated by Hedgehog signalling from the notochord. Our findings suggest a hypothesis for ancestral vertebrate trunk myogenic patterning and how it was co-opted during tail evolution to generate similar muscle by new mechanisms. This article has an associated ‘The people behind the papers’ interview.
format Online
Article
Text
id pubmed-7197714
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Company of Biologists Ltd
record_format MEDLINE/PubMed
spelling pubmed-71977142020-05-14 Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis Osborn, Daniel P. S. Li, Kuoyu Cutty, Stephen J. Nelson, Andrew C. Wardle, Fiona C. Hinits, Yaniv Hughes, Simon M. Development Research Article Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived Fgf signals act through Tbx16 and Tbxta to induce slow and fast trunk muscle precursors at distinct dorsoventral positions. Tbx16 binds to and directly activates the myf5 and myod genes, which are required for commitment to myogenesis. Tbx16 activity depends on Fgf signalling from the organiser. In contrast, Tbxta is not required for myf5 expression, but binds a specific site upstream of myod that is not bound by Tbx16 and drives (dependent on Fgf signals) myod expression in adaxial slow precursors, thereby initiating trunk myogenesis. After gastrulation, when similar muscle cell populations in the post-anal tail are generated from tailbud, declining Fgf signalling is less effective at initiating adaxial myogenesis, which is instead initiated by Hedgehog signalling from the notochord. Our findings suggest a hypothesis for ancestral vertebrate trunk myogenic patterning and how it was co-opted during tail evolution to generate similar muscle by new mechanisms. This article has an associated ‘The people behind the papers’ interview. The Company of Biologists Ltd 2020-04-28 /pmc/articles/PMC7197714/ /pubmed/32345657 http://dx.doi.org/10.1242/dev.184689 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Osborn, Daniel P. S.
Li, Kuoyu
Cutty, Stephen J.
Nelson, Andrew C.
Wardle, Fiona C.
Hinits, Yaniv
Hughes, Simon M.
Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
title Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
title_full Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
title_fullStr Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
title_full_unstemmed Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
title_short Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
title_sort fgf-driven tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197714/
https://www.ncbi.nlm.nih.gov/pubmed/32345657
http://dx.doi.org/10.1242/dev.184689
work_keys_str_mv AT osborndanielps fgfdriventbxproteinactivitiesdirectlyinducemyf5andmyodtoinitiatezebrafishmyogenesis
AT likuoyu fgfdriventbxproteinactivitiesdirectlyinducemyf5andmyodtoinitiatezebrafishmyogenesis
AT cuttystephenj fgfdriventbxproteinactivitiesdirectlyinducemyf5andmyodtoinitiatezebrafishmyogenesis
AT nelsonandrewc fgfdriventbxproteinactivitiesdirectlyinducemyf5andmyodtoinitiatezebrafishmyogenesis
AT wardlefionac fgfdriventbxproteinactivitiesdirectlyinducemyf5andmyodtoinitiatezebrafishmyogenesis
AT hinitsyaniv fgfdriventbxproteinactivitiesdirectlyinducemyf5andmyodtoinitiatezebrafishmyogenesis
AT hughessimonm fgfdriventbxproteinactivitiesdirectlyinducemyf5andmyodtoinitiatezebrafishmyogenesis