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Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis
Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197714/ https://www.ncbi.nlm.nih.gov/pubmed/32345657 http://dx.doi.org/10.1242/dev.184689 |
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author | Osborn, Daniel P. S. Li, Kuoyu Cutty, Stephen J. Nelson, Andrew C. Wardle, Fiona C. Hinits, Yaniv Hughes, Simon M. |
author_facet | Osborn, Daniel P. S. Li, Kuoyu Cutty, Stephen J. Nelson, Andrew C. Wardle, Fiona C. Hinits, Yaniv Hughes, Simon M. |
author_sort | Osborn, Daniel P. S. |
collection | PubMed |
description | Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived Fgf signals act through Tbx16 and Tbxta to induce slow and fast trunk muscle precursors at distinct dorsoventral positions. Tbx16 binds to and directly activates the myf5 and myod genes, which are required for commitment to myogenesis. Tbx16 activity depends on Fgf signalling from the organiser. In contrast, Tbxta is not required for myf5 expression, but binds a specific site upstream of myod that is not bound by Tbx16 and drives (dependent on Fgf signals) myod expression in adaxial slow precursors, thereby initiating trunk myogenesis. After gastrulation, when similar muscle cell populations in the post-anal tail are generated from tailbud, declining Fgf signalling is less effective at initiating adaxial myogenesis, which is instead initiated by Hedgehog signalling from the notochord. Our findings suggest a hypothesis for ancestral vertebrate trunk myogenic patterning and how it was co-opted during tail evolution to generate similar muscle by new mechanisms. This article has an associated ‘The people behind the papers’ interview. |
format | Online Article Text |
id | pubmed-7197714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-71977142020-05-14 Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis Osborn, Daniel P. S. Li, Kuoyu Cutty, Stephen J. Nelson, Andrew C. Wardle, Fiona C. Hinits, Yaniv Hughes, Simon M. Development Research Article Skeletal muscle derives from dorsal mesoderm formed during vertebrate gastrulation. Fibroblast growth factor (Fgf) signalling cooperates with Tbx transcription factors to promote dorsal mesoderm formation, but their role in myogenesis has been unclear. Using zebrafish, we show that dorsally derived Fgf signals act through Tbx16 and Tbxta to induce slow and fast trunk muscle precursors at distinct dorsoventral positions. Tbx16 binds to and directly activates the myf5 and myod genes, which are required for commitment to myogenesis. Tbx16 activity depends on Fgf signalling from the organiser. In contrast, Tbxta is not required for myf5 expression, but binds a specific site upstream of myod that is not bound by Tbx16 and drives (dependent on Fgf signals) myod expression in adaxial slow precursors, thereby initiating trunk myogenesis. After gastrulation, when similar muscle cell populations in the post-anal tail are generated from tailbud, declining Fgf signalling is less effective at initiating adaxial myogenesis, which is instead initiated by Hedgehog signalling from the notochord. Our findings suggest a hypothesis for ancestral vertebrate trunk myogenic patterning and how it was co-opted during tail evolution to generate similar muscle by new mechanisms. This article has an associated ‘The people behind the papers’ interview. The Company of Biologists Ltd 2020-04-28 /pmc/articles/PMC7197714/ /pubmed/32345657 http://dx.doi.org/10.1242/dev.184689 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Osborn, Daniel P. S. Li, Kuoyu Cutty, Stephen J. Nelson, Andrew C. Wardle, Fiona C. Hinits, Yaniv Hughes, Simon M. Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis |
title | Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis |
title_full | Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis |
title_fullStr | Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis |
title_full_unstemmed | Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis |
title_short | Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis |
title_sort | fgf-driven tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197714/ https://www.ncbi.nlm.nih.gov/pubmed/32345657 http://dx.doi.org/10.1242/dev.184689 |
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