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Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale

Cultivation of yeast Pichia pastoris in the microtiter plate, for optimisation of culture conditions, and expression screening of transformants has gained significance in recent years. However, in the microtiter plate, it has been challenging to attain cell densities similar to well-aerated shake-fl...

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Detalles Bibliográficos
Autores principales: Kaushik, Neha, Lamminmäki, Urpo, Khanna, Navin, Batra, Gaurav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198582/
https://www.ncbi.nlm.nih.gov/pubmed/32366873
http://dx.doi.org/10.1038/s41598-020-63995-5