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Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale
Cultivation of yeast Pichia pastoris in the microtiter plate, for optimisation of culture conditions, and expression screening of transformants has gained significance in recent years. However, in the microtiter plate, it has been challenging to attain cell densities similar to well-aerated shake-fl...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198582/ https://www.ncbi.nlm.nih.gov/pubmed/32366873 http://dx.doi.org/10.1038/s41598-020-63995-5 |